Methods for using a pipetting device with independently movable pipette units

Patent metadata

Field	Value
Publication number	US-9244087-B2
Application number	US-201514792155-A
Country	US
Kind code	B2
Filing date	Jul 6, 2015
Priority date	Jun 29, 2010
Publication date	Jan 26, 2016
Grant date	Jan 26, 2016

Abstract

The invention provides for a method of pipetting samples using a pipetting device including more than one pipetting unit. Each of the pipetting units are independently movable in Y and Z directions and includes an upper and a lower frame. Two adjacent pipetting units include a module arranged in a staggered manner in either of the respective upper and lower frames.

First claim

What is claimed is:

1. A method of isolating and purifying an analyte present in at least one liquid samples in an automated analytical system, comprising the automated steps of
providing a first set of vessels comprising said samples to said automated analytical system;
aspirating at least a portion of said at least two liquid samples from said first set of vessels with a pipetting device comprising more than one pipetting unit, wherein a distance between said pipetting units is adjusted to the a first distance between the first set of vessels prior to aspiration;
adjusting the distance between said pipetting units to a second distance, different than the first distance, between vessels of a second set of vessels;
dispensing said liquid samples into said second set of vessels;
combining together a solid support material and liquid samples in said second set of vessels for a period of time and under conditions sufficient to permit said analyte to be immobilized on the solid support material;
isolating said analyte immobilized on the solid support material in a separation station; and
purifying said analyte in the separation station by separating the solid support material from the liquid samples and washing the solid support material one or more times with a wash buffer wherein said analyte is a nucleic acid.

2. The method of claim 1 , wherein said pipetting device comprises: a main body comprising a receiving part; two or more adjacent pipetting units extending vertically parallel to each other, each of said pipetting units comprising: an interacting part releasably engaging the receiving part of the main body in a fixed position; a pipette tip; an interface for interacting with an interface of the pipette tip; an upper frame and a lower frame each configured to receive and mount a module, at least one module present in the upper or lower frame of each pipetting unit, wherein the modules of two adjacent pipetting units are mounted in a staggered fashion between the upper frame of one of the two or more adjacent pipetting units, and the lower frame of a second adjacent pipetting unit; and an X-transfer mechanism connected to said main body to which said two or more adjacent pipetting units are connected.

3. The method of claim 2 , wherein said modules comprise at least one of: a pump for aspirating and dispensing a liquid; a Y-axis actuator, and a Z-axis actuator, wherein said Y-axis actuator and said Z-axis actuator function independently; or an electronic module for controlling functions of said two or more pipetting adjacent units.

Assignees

• D Amore Alessandro
• Knecht Urs
• Schneebeli Rolf
• Roche Molecular Systems Inc

Inventors

• D Amore Alessandro
• Knecht Urs
• Schneebeli Rolf

Classifications

• G01N35/1067 Primary

  for transfer to or from containers having different spacing · CPC title

• G01N35/1016

  Control of the volume dispensed or introduced · CPC title

• B01L3/021

  Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids · CPC title

• G01N35/1011 Primary

  Control of the position or alignment of the transfer device · CPC title