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US-2024404593-A1 · Dec 5, 2024 · US
Droplet-based pyrosequencing
US9243282B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9243282-B2 |
| Application number | US-201213470919-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 14, 2012 |
| Priority date | Apr 18, 2006 |
| Publication date | Jan 26, 2016 |
| Grant date | Jan 26, 2016 |
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Abstract
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The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets include reagents for extending a double stranded portion of the sample nucleic acid by incorporating a nucleotide at the target position; (ii) splitting the second droplet to yield a third droplet including the bead and a fourth droplet lacking the bead; and (iii) assaying the third droplet to determine whether the nucleotide was incorporated at the target position.
First claim
Opening claim text (preview).
We claim: 1. A method of identifying a base at a target position in a sample nucleic acid, the method comprising: (a) providing a droplet microactuator comprising a first droplet comprising a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to yield a second droplet, wherein the one or more reagent droplets comprise reagents for extending a double stranded portion of the sample n…
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Classifications
- C12Q1/68Primary
Chemistry & Metallurgy · mapped topic
- C12Q1/6869Primary
Chemistry & Metallurgy · mapped topic
Operations & Transport · mapped topic
Operations & Transport · mapped topic
Operations & Transport · mapped topic
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- What does patent US9243282B2 cover?
- The present invention relates to droplet-based pyrosequencing including a method of identifying a base at a target position in a sample nucleic acid. The method includes: (a) providing a droplet microactuator including a first droplet including a sample nucleic acid immobilized on a bead; and (b) on the droplet microactuator: (i) contacting the first droplet with one or more reagent droplets to…
- Who is the assignee on this patent?
- Pollack Michael G, Pamula Vamsee K, Fair Richard B, and 4 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/68. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 26 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).