Effervescent compositions and uses thereof

Patent metadata

Field	Value
Publication number	US-9243279-B2
Application number	US-201113989922-A
Country	US
Kind code	B2
Filing date	Dec 22, 2011
Priority date	Dec 31, 2010
Publication date	Jan 26, 2016
Grant date	Jan 26, 2016

Abstract

A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.

First claim

The invention claimed is:

1. A method of enriching the growth of a target microorganism, comprising:
providing a culture medium, a sample that may include the target microorganism, and a latent effervescent body;
wherein the latent effervescent body comprises an outer shell with a core composition disposed therein
wherein the core composition comprises two or more effervescent components and a selective agent that facilitates the growth of the target microorganism relative to growth of at least one other microorganism;
wherein the core composition comprises a lithium compound that functions as one of the effervescent components and as the selective agent;

contacting the sample, the latent effervescent body, and the culture medium under conditions to facilitate growth of the target microorganism; and
releasing the selective agent from the latent effervescent body;
wherein releasing the selective agent further comprises releasing the selective agent after an aqueous mixture comprising the sample and the culture medium are placed in fluidic contact with the latent effervescent body for a predetermined period of time;
wherein the predetermined time is about 2 hours to about 6 hours.

2. The method of claim 1 , wherein releasing the selective agent further comprises distributing the selective agent in the culture medium.

3. The method of claim 2 , wherein distributing the selective agent further comprises distributing the selective agent via a mixing process resulting from gaseous effervescence.

4. The method of claim 1 , wherein the predetermined time is less than 2 hours.

5. The method of claim 1 , further comprising detecting a microorganism.

6. The method of claim 5 , wherein detecting the microorganism comprises propagating the microorganism on or in a semisolid culture medium, observing the microorganism microscopically, detecting metabolic activity of the microorganism, detecting a biomolecule of the microorganism, or detecting a nucleic acid of the microorganism.

7. A latent effervescent body, comprising;
an outer shell that disintegrates when contacted with an aqueous liquid; and
a core composition disposed therein;
wherein the core composition comprises first and second effervescent components, a disintegration aid, and a selective agent that facilitates growth of a target microorganism relative to growth of at least one other microorganisms;
wherein the disintegration aid comprises a water-swellable polymer;
wherein the core composition comprises a lithium compound that functions as one of the effervescent components and as the selective agent.

8. The latent effervescent body of claim 7 , further comprising a flow agent, a buffer component, a microbial growth indicator, a nutrient, a binder, a lubricant, a filler, an anti-adhesion agent, or a combination of any two or more of the foregoing.

9. The latent effervescent body of claim 8 , wherein the microbial growth indicator comprises a pH indicator, an enzyme substrate, or a redox indicator.

10. The latent effervescent body of claim 7 , wherein the outer shell comprises polyvinyl alcohol, poly(meth)acrylate, cellulose and methylcellulose, propylcellulose, derivatives of any of the foregoing, or mixtures of any of the foregoing.

11. The latent effervescent body of claim 7 , wherein the core composition comprises a shaped composition.

12. The latent effervescent body of claim 7 , wherein the outer shell comprises a pouch.

13. The latent effervescent body of claim 12 , wherein the outer shell conforms to the shape of the shaped composition.

14. The latent effervescent body of claim 7 , wherein the first effervescent component comprises a base and the second effervescent component comprises an acid.

15. The latent effervescent body of claim 7 , wherein the first effervescent component comprises lithium carbonate, lithium bicarbonate, or a mixture thereof.

16. The latent effervescent body of claim 7 , wherein the second effervescent component comprises citric acid, tartaric acid, itaconic acid, or a mixture of any two or more of the foregoing.

17. The latent effervescent body of claim 7 , wherein the selective agent comprises a salt, an antibiotic, a surfactant, a dye, or a bacteriocin.

18. The latent effervescent body of claim 17 , wherein the selective agent is selected from the group consisting of novobiocin, penicillin, acriflavin HCl, naladixic acid, cycloheximide, amphotericin B, ceftazidime hydrate, streptomycin, ampicillin, colistin, nisin, tetracycline, phenylethyl alcohol, sodium desoxycholate, sodium dodecylsulfate, bile salts, ox gall, sodium thiosulfate, NaCl, lithium chloride, lithium carbonate, methylene blue, brilliant green dye, crystal violet, and Malachite green.

Assignees

• Xia Wensheng
• Mach Patrick A
• Beaurline Joseph M
• Bjork Jason W
• Liu Jie J
• 3M Innovative Properties Co

Inventors

• Xia Wensheng
• Mach Patrick A
• Beaurline Joseph M
• Bjork Jason W
• Liu Jie J

Classifications

• C12Q1/04 Primary

  Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor {(C12Q1/6897 takes precedence)} · CPC title

• C12N1/20

  Bacteria; Culture media therefor · CPC title

• C12N1/14 Primary

  Fungi (culture of mushrooms A01G18/00; as new plants A01H15/00); Culture media therefor · CPC title

• C12Q1/045 Primary

  Culture media therefor · CPC title

• C12Q1/689

  for bacteria · CPC title