Glucosyl stevia composition
US-2015157045-A1 · Jun 11, 2015 · US
Method for making rebaudioside X
US9243273B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9243273-B2 |
| Application number | US-201414469076-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 26, 2014 |
| Priority date | May 22, 2012 |
| Publication date | Jan 26, 2016 |
| Grant date | Jan 26, 2016 |
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Abstract
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Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and X are described. The method includes expression of UDP-glucosyltransferases from Stevia rebaudiana Bertoni, which are capable converting certain steviol glycosides to rebaudiosides A, D and X. The highly purified rebaudiosides A, D and X, are useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakery products, cookies, and chewing gums.
First claim
Opening claim text (preview).
We claim: 1. A method for making Rebaudioside X comprising a step of converting Rebaudioside D to Rebaudioside X using a UDP-glucosyltransferase, wherein the conversion of Rebaudioside D to Rebaudioside X is at least about 50% complete. 2. The method of claim 1 , wherein the UDP-glucosyltransferase comprises any UDP-glucosyltransferase capable of adding at least one glucose unit to Rebaudioside D to form Rebaudioside X. 3. The method of claim 2 , wherein the UDP-glucosyltransferase is capable of adding a glucose unit to a C-19 position of Rebaudioside D. 4. The method of claim 1 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 80% by weight. 5. The method of claim 1 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 90% by weight. 6. The method of claim 1 , further comprising the step of purifying the Rebaudioside X to a purity of greater than about 95% by weight. 7. The method of claim 1 , wherein the conversion is at least about 60% complete. 8. The method of claim 1 , wherein the conversion is at least about 70% complete. 9. The method of claim 1 , wherein the conversion is at least about 80% complete. 10. The method of claim 1 , wherein the conversion is at least about 90% complete. 11. The method of claim 1 , wherein the conversion is at least about 95% complete. 12. The method of claim 1 , wherein the UDP-glucosyltransferase is expressed in a host microorganism. 13. The method of claim 12 , wherein the UDP-glucosyltransferase host microorganism is selected from the group consisting of: Escherichia coli, Saccharomyces species, Aspergillus species, and Pischia species. 14. The method of claim 1 , wherein the UDP-glucosyltransferase comprises UGT76G1.
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for hyperglycaemia, e.g. antidiabetics · CPC title
Recycling of unreacted starting or intermediate materials · CPC title
- C12P19/56Primary
having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin · CPC title
Condensed ring systems having three or more rings · CPC title
- A23L27/36Primary
Terpene glycosides · CPC title
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- What does patent US9243273B2 cover?
- Methods of preparing highly purified steviol glycosides, particularly rebaudiosides A, D and X are described. The method includes expression of UDP-glucosyltransferases from Stevia rebaudiana Bertoni, which are capable converting certain steviol glycosides to rebaudiosides A, D and X. The highly purified rebaudiosides A, D and X, are useful as non-caloric sweetener in edible and chewable comp…
- Who is the assignee on this patent?
- Purecircle Sdn Bhd, Coca Cola Co
- What technology area does this patent fall under?
- Primary CPC classification C12P19/56. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jan 26 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 2 related publications on this page (citations in our corpus or others sharing the same primary CPC).