Vesicles and nanostructures from recombinant proteins

Patent metadata

Field	Value
Publication number	US-9238010-B2
Application number	US-201213976796-A
Country	US
Kind code	B2
Filing date	Jan 13, 2012
Priority date	Jan 13, 2011
Publication date	Jan 19, 2016
Grant date	Jan 19, 2016

Abstract

The present invention includes a composition comprising at least one oleosin-like protein. The present invention also includes a composition comprising a vesicle comprising at least one oleosin-like protein.

First claim

What is claimed is:

1. A composition comprising a protein comprising a sequence that has at least 80% homology to an oleosin-like protein selected from the group consisting of SEQ ID NOs:21-26, and combinations thereof, wherein said protein is capable of self-assembling into a polypeptide vesicle.

2. The composition of claim 1 , wherein said sequence has at least 90% homology to an oleosin-like protein selected from the group consisting of SEQ ID NOs:21-26, and combinations thereof.

3. The composition of claim 2 , wherein said sequence has at least 95% homology to an oleosin-like protein selected from the group consisting of SEQ ID NOs:21-26, and combinations thereof.

4. The composition of claim 1 , wherein a receptor binding motif is attached to the N-terminus or the C-terminus of said sequence via an amide bond.

5. The composition of claim 4 , wherein said motif is selected from the group consisting of SEQ ID NOs:4-6.

6. The composition of claim 1 , wherein at least two residues of said oleosin-like protein are replaced with cysteine residues.

7. The composition of claim 1 , wherein at least one tyrosine residue in said oleosin-like protein is converted to a L-DOPA residue via a chemical reaction.

8. The composition of claim 7 , wherein at least two of said L-DOPA residues are further crosslinked via a chemical reaction.

9. The composition of claim 1 , wherein at least one sequence selected from the group consisting of SEQ ID NOs:8-12 is inserted in said oleosin-like protein (i) at the junction of hydrophilic and hydrophobic segments, or (ii) within the hydrophilic segment.

10. A composition comprising a vesicle, wherein said vesicle comprises a protein comprising a sequence that has at least 80% homology to an oleosin-like protein selected from the group consisting of SEQ ID NOs:21-26, and combinations thereof.

11. The composition of claim 10 , further comprising a compound encapsulated within said vesicle, wherein said compound is selected from the group consisting of a gas, drug, fluorescent dye, radioactive probe, salt, protein, and nucleic acid.

12. The composition of claim 10 , further comprising at least one pharmaceutically acceptable carrier.

13. A composition comprising a protein comprising a peptide sequence as set forth in residues 26-88 of SEQ ID NO:21, wherein said protein is capable of self-assembling into a polypeptide vesicle.

Assignees

• Hammer Daniel A
• Vargo Kevin
• Parthasarathy Ranganath
• Univ Pennsylvania

Inventors

• Hammer Daniel A
• Vargo Kevin
• Parthasarathy Ranganath

Classifications

• A61K9/1272 Primary

  comprising non-phosphatidyl surfactants as bilayer-forming substances, e.g. cationic lipids or non-phosphatidyl liposomes coated or grafted with polymers (lipids as modifying agents {A61K47/543}) · CPC title

• A61K9/5169 Primary

  Proteins, e.g. albumin, gelatin · CPC title

• A61K38/168

  from plants · CPC title

• A61K45/06

  Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca · CPC title

• A61K38/00

  Medicinal preparations containing peptides (peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00) · CPC title