Magnetic immunosensor and method of use

Patent metadata

Field	Value
Publication number	US-9233370-B2
Application number	US-201113204121-A
Country	US
Kind code	B2
Filing date	Aug 5, 2011
Priority date	Aug 5, 2010
Publication date	Jan 12, 2016
Grant date	Jan 12, 2016

Abstract

The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field.

First claim

What is claimed is:

1. A microfabricated magnetic layer on a substantially planar surface, wherein the microfabricated magnetic layer comprises: high-field permanent magnetic particulates dispersed in a thermally, chemically or photoformably cured immobilization matrix; wherein the immobilization matrix comprises a polyimide, polyvinyl alcohol, or epoxy resin;
wherein the microfabricated magnetic layer is positioned substantially proximate to a microfabricated sensing electrode,
wherein the electrode is connected by a conductive line to instrument electronics, wherein the microfabricated magnetic layer is not the conductive line,
wherein the microfabricated magnetic layer is positioned to attract magnetically susceptible beads coated with a capture antibody to a target analyte,
wherein the electrode is an amperometric electrode, and
wherein the magnetic layer is configured to concentrate the magnetically susceptible beads with the capture antibody and the target analyte within a sample fluid at the electrode for measurement, and
wherein the amperometric electrode is configured to detect the target analyte.

2. The microfabricated magnetic layer of claim 1 , wherein the magnetic particulates comprise a neodymium iron boron (NdFeB) alloy.

3. The microfabricated magnetic layer of claim 1 , wherein the magnetic particulates comprise a Nd 2 Fe 14 B alloy.

4. The microfabricated magnetic layer of claim 1 , wherein the magnetic particulates have an average particle size of from 0.01 μm to 20 μm.

5. The microfabricated magnetic layer of claim 1 , wherein the magnetic particulates have an average particle size of from 0.01 μm to 5 μm.

6. The microfabricated magnetic layer of claim 1 , wherein the immobilization matrix comprises polyimide.

7. The microfabricated magnetic layer of claim 1 , wherein the immobilization matrix comprises polyvinyl alcohol.

8. The microfabricated magnetic layer of claim 1 , wherein the magnetic particulates provide a magnetic field of greater than about 0.1 Tesla.

9. The microfabricated magnetic layer of claim 1 , wherein the microfabricated magnetic layer is positioned to yield an event horizon for the magnetically susceptible beads in the range of less than about 200 μm in the region of the electrode.

10. The microfabricated magnetic layer of claim 1 , wherein the electrode is a gold microarray.

11. The microfabricated magnetic layer of claim 1 , wherein the microfabricated magnetic layer is positioned over the substantially planar surface.

12. The microfabricated magnetic layer of claim 1 , wherein the microfabricated magnetic layer is directly attached to the substantially planar surface.

13. The microfabricated magnetic layer of claim 1 , wherein the microfabricated magnetic layer is coated onto the surface of the substantially planar surface.

14. The microfabricated magnetic layer of claim 1 , wherein the microfabricated magnetic layer is patterned onto the surface of the substantially planar surface.

15. The microfabricated magnetic layer of claim 1 , wherein the microfabricated magnetic layer is positioned proximate to the sensing electrode.

Assignees

• Miller Cary James
• Hu Jinghua
• Abbott Point Of Care Inc

Inventors

• Miller Cary James
• Hu Jinghua

Classifications

• G01N33/54326 Primary

  Magnetic particles · CPC title

• G01N27/745

  for detecting magnetic beads used in biochemical assays (concerning the assays G01N33/54326; sensors therefor G01R33/1269; automatic analysers therefor G01N35/0098) · CPC title

• B01L3/502761 Primary

  specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules · CPC title

• G01N33/5438

  Electrodes · CPC title

• B01L2300/0645

  Electrodes · CPC title