Spatial positioning of spectrally labeled beads

Patent metadata

Field	Value
Publication number	US-9228948-B2
Application number	US-201514700248-A
Country	US
Kind code	B2
Filing date	Apr 30, 2015
Priority date	Sep 24, 1998
Publication date	Jan 5, 2016
Grant date	Jan 5, 2016

Abstract

Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser.

First claim

What is claimed is:

1. A method comprising:
restraining a plurality of spectrally labeled bodies with affinity binding so as to define a multiplexed array, wherein the spectrally labeled bodies are spatially resolved and comprise a plurality of fluorescent labels used to encode discrete and different emission spectra wherein each emission spectrum has a plurality of signals at differing wavelengths;
directing an image of the multiplexed array of bodies onto a sensor to sense spectra generated by the bodies; and
identifying the bodies from the spectra sensed by the sensor.

2. The method of claim 1 , wherein the bodies are affixed to each other.

3. The method of claim 1 , wherein the bodies are restrained within an array of openings affixed in a support structure.

4. The method of claim 1 , wherein the sensor is a CCD camera.

5. A method of claim 1 , wherein the spectrally labeled bodies include polymers.

6. A method of claim 1 , wherein affinity binding is facilitated by a chemical moiety.

7. A method of claim 1 , wherein the affinity binding is specific.

8. A method of claim 1 , wherein the plurality of signals include a reference signal.

9. A method of claim 1 , wherein the affinity binding is facilitated with a binding pair.

10. The method of claim 1 , wherein the bodies are restrained to a support structure.

11. The method of claim 1 , wherein the bodies are restrained in the multiplexed array by an array of discrete binding sites.

12. A method of claim 8 , wherein the reference signal identifies a spectral code family.

13. A method of claim 9 , wherein one member of the binding pair is affixed to at least one of the plurality of spectrally labeled bodies and the other member is affixed to a site of the multiplexed array.

14. The method of claim 10 , wherein the support structure has a substantially planar surface.

15. The method of claim 10 , wherein the support structure contains a plurality of openings sized to accommodate one of the spectrally labeled bodies.

16. The method of claim 10 , wherein a plurality of unbound bodies are washed away.

17. A method of claim 11 , wherein the discrete binding sites are arranged so as to inhibit a presence of more than a single body at each binding site.

18. A method of claim 11 , wherein the binding sites comprise a material capable of binding to the bodies.

19. A method of claim 18 , wherein the material comprises a selective affinity molecule which selectively binds to one or more particular bodies.

20. The method of claim 19 , wherein the selective affinity molecule is streptavidin.

21. The method of claim 19 , wherein a selective binding material is a biotinylated structure.

Assignees

• Life Technologies Corp

Inventors

• Empedocles Stephen
• Watson Andrew
• Jin Jian

Classifications

• B01L3/5025 Primary

  for parallel transport of multiple samples · CPC title

• G01N2035/00742

  Type of codes · CPC title

• G01N2015/1472

  with colour · CPC title

• G01N21/6452

  Individual samples arranged in a regular 2D-array, e.g. multiwell plates · CPC title

• B01J2219/00578

  electrophoric · CPC title