Biomaterial scaffolds with keratin for tissue engineering

Patent metadata

Field	Value
Publication number	US-9226993-B2
Application number	US-21671808-A
Country	US
Kind code	B2
Filing date	Jul 10, 2008
Priority date	Jul 10, 2008
Publication date	Jan 5, 2016
Grant date	Jan 5, 2016

Abstract

The present invention relates to methods for preparing porous natural or synthetic material scaffolds with keratin for improving cell affinity.

First claim

The invention claimed is:

1. A method for combining a cell adhesion-enhancing keratin with a biomaterial scaffold, comprising the steps of:
(a) mixing keratin with a biomaterial to form a keratin-biomaterial solution;
(b) mixing porogen with said keratin-biomaterial solution to form a body;
(c) drying said body; and
(d) dissolving and leaching out the porogen from said body.

2. The method of claim 1 , wherein said method is a continuous process.

3. The method of claim 1 , wherein said method is a batch process.

4. The method of claim 1 , wherein said keratin includes a liquid.

5. The method of claim 4 , wherein said keratin is at least one of a solution, a suspension, a slurry, flowable powder, flowable fiber, flowable paste, and mixtures thereof.

6. The method of claim 1 , wherein said porogen is selected from the group consisting of sodium chloride, sodium sulfate, potassium chloride, sodium iodide, sodium fluoride, potassium fluoride, sodium nitrate, sodium iodate, sodium hydroxide, fructose, saccharin, glucose, paraffin, beeswax, naphthalene, and gelatin.

7. The method of claim 6 , wherein said porogen after being mixed with said keratin-biomaterial solution is formed into a shape of a cubic, geometrically-shaped crystals, geometric shapes, or mixtures thereof.

8. The method of claim 1 , wherein said biomaterial is selected from group consisting of poly(lactide-co-glycolide) (PLGA), poly(lactide) (PLLA), polyglycolic acid (PGA), polyanhydrides, poly(ortho ethers), poly caprolactone, polyethylene glycol (PEG), polyurethane, copolymers thereof, and mixtures thereof.

9. The method of claim 1 , wherein said keratin is fibrous or powdered.

10. The method of claim 1 , further comprising the step, after mixing keratin with biomaterial solution, adding functional or inert additives to the solution.

Assignees

• Li Yi
• Li Jiashen
• Hu Junyan
• Li Lin
• Univ Hong Kong Polytechnic

Inventors

• Li Yi
• Li Jiashen
• Hu Junyan
• Li Lin

Classifications

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• A61L27/227 Primary

  Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24 · CPC title

• A61L27/18

  obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds · CPC title

• C08L67/04

  Polyesters derived from hydroxycarboxylic acids, e.g. lactones (C08L67/06 takes precedence) · CPC title