Rodenticide

Patent metadata

Field	Value
Publication number	US-9220263-B2
Application number	US-74215308-A
Country	US
Kind code	B2
Filing date	Nov 10, 2008
Priority date	Nov 8, 2007
Publication date	Dec 29, 2015
Grant date	Dec 29, 2015

Abstract

The present invention relates to rodenticides, methods of killing a rodent comprising administering to said rodent, a lethal dose of an isolated Yersinia murine toxin polypeptide or an isolated antigenic analogue thereof, and methods of manufacturing novel rodenticides.

First claim

The invention claimed is:

1. A method of killing a rodent, comprising administering to the rodent a lethal dose of isolated Yersinia murine toxin polypeptide or an isolated antigenic analogue thereof, wherein the Yersinia murine toxin polypeptide or the isolated antigenic analogue thereof is administered in a feed, and wherein administration of the Yersinia murine toxin polypeptide or isolated antigenic analogue thereof in the feed kills the rodent to which it is administered
wherein the isolated antigenic analogue is a polypeptide that is capable of killing a rodent in a similar dose dependence as isolated Yersinia murine toxin polypeptide and shares more than 60% identity or similarity with SEQ ID NO:2.

2. The method of claim 1 , wherein the rodent is a rat.

3. The method of claim 2 , wherein the rat is of the species Rattus rattus or Rattus norvegicus.

4. The method of claim 1 , wherein the isolated Yersinia murine toxin polypeptide or the isolated antigenic analogue thereof is a recombinant Yersinia murine toxin polypeptide or a recombinant antigenic analogue thereof.

5. The method of claim 1 , wherein the Yersinia murine toxin polypeptide or the isolated antigenic analogue is administered in the presence of one or more protease inhibitors.

6. The method of claim 1 , wherein the Yersinia murine toxin polypeptide or the isolated antigenic analogue additionally comprises a HIS tag.

7. The method of claim 1 , wherein the isolated Yersinia murine toxin polypeptide or the isolated antigenic analogue thereof is a recombinant Yersinia murine toxin or its antigenic analogue obtained by a method comprising the steps of:
putting a polynucleotide encoding the Yersinia murine toxin or its antigenic analogue in an expression plasmid;
transforming the expression plasmid into an expression host organism;
expressing the Yersinia murine toxin or its antigenic analogue in the expression host organism; and
purifying the Yersinia murine toxin or its antigenic analogue.

8. The method of claim 7 , wherein the expression plasmid additionally encodes a polypeptide tag that is attached to the Yersinia murine toxin or its antigenic analogue.

9. The method of claim 8 , wherein the polypeptide tag is a GST or HIS tag.

10. The method of claim 9 , wherein the polypeptide tag is a HIS tag.

11. The method of claim 8 , wherein the method additionally comprises the step of cleaving the tag after purification has taken place.

12. A method of killing a rodent comprising administering to the rodent a lethal dose of isolated Yersinia murine toxin polypeptide or an isolated antigenic analogue thereof, wherein the Yersinia murine toxin polypeptide or the isolated antigenic analogue thereof is administered by oral administration, and wherein oral administration of the Yersinia murine toxin polypeptide or isolated antigenic analogue thereof kills the rodent to which it is administered
wherein the isolated antigenic analogue is a polypeptide that is capable of killing a rodent in a similar dose dependence as isolated Yersinia murine toxin polypeptide and shares more than 60% identity or similarity with SEQ ID NO:2.

13. The method of claim 12 , wherein the rodent is a rat.

14. The method of claim 13 , wherein the rat is of the species Rattus rattus or Rattus norvegicus.

15. The method of claim 12 , wherein the isolated Yersinia murine toxin polypeptide or the isolated antigenic analogue thereof is a recombinant Yersinia murine toxin polypeptide or a recombinant antigenic analogue thereof.

16. The method of claim 12 , wherein the Yersinia murine toxin polypeptide or the isolated antigenic analogue is administered in the presence of one or more protease inhibitors.

17. The method of claim 12 , wherein the Yersinia murine toxin polypeptide or the isolated antigenic analogue additionally comprises a HIS tag.

18. The method of claim 12 , wherein the isolated Yersinia murine toxin polypeptide or the isolated antigenic analogue thereof is a recombinant Yersinia murine toxin or its antigenic analogue obtained by a method comprising the steps of:
putting a polynucleotide encoding the Yersinia murine toxin or its antigenic analogue in an expression plasmid;
transforming the expression plasmid into an expression host organism;
expressing the Yersinia murine toxin or its antigenic analogue in the expression host organism; and
purifying the Yersinia murine toxin or its antigenic analogue.

19. The method of claim 18 , wherein the expression plasmid additionally encodes a polypeptide tag that is attached to the Yersinia murine toxin or its antigenic analogue.

20. The method of claim 19 , wherein the polypeptide tag is a GST or HIS tag.

21. The method of claim 20 , wherein the polypeptide tag is a HIS tag.

22. The method of claim 18 , wherein the method additionally comprises the step of cleaving the tag after purification has taken place.

23. The method of claim 1 , wherein the isolated antigenic analogue shares more than 90% identity or similarity with SEQ ID NO:2.

24. The method of claim 12 , wherein the isolated antigenic analogue shares more than 90% identity or similarity with SEQ ID NO:2.

Assignees

• Oyston Petra Claire Farquhar
• Clark Graeme Christopher
• Secr Defence

Inventors

• Oyston Petra Claire Farquhar
• Clark Graeme Christopher

Classifications

• C07K14/24

  from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia · CPC title

• A61K9/5005

  Wall or coating material · CPC title

• A01N37/46 Primary

  N-acyl derivatives · CPC title

• A01N63/00

  Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates (containing compounds of determined constitution A01N27/00 - A01N59/00; unicellular algae A01N65/03) · CPC title

• A01N63/02

  Human Necessities · mapped topic