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Compositions and methods for detecting nucleic acid from mollicutes
US9212397B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9212397-B2 |
| Application number | US-82160810-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 23, 2010 |
| Priority date | Jun 23, 2009 |
| Publication date | Dec 15, 2015 |
| Grant date | Dec 15, 2015 |
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Abstract
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Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing regions, promoter sequences, and/or binding moieties. Samples can be from any source suspected of containing a species of the class Mollicutes. Preferred sample sources include bioreactors, cell lines, cell culture wares and pharmaceutical manufacturing wares.
First claim
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The invention claimed is: 1. A method for the in vitro amplification and detection of a nucleic acid in a sample, said nucleic acid being from one or more species in the class Mollicutes, comprising the steps of: (a.) contacting a sample with at least four tagged amplification oligomers and at least one additional amplification oligomer for generating amplification product from one or more nucleic acids in said sample, wherein each of said at least four tagged amplification oligom…
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- C12Q1/689Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9212397B2 cover?
- Compositions, reaction mixtures, kits and methods used in amplifying and detecting nucleic acids from various species of the class Mollicutes. Particular regions of the 23S rRNA or its gene have been identified as preferred targets for nucleic acid amplification reactions of a sample suspected containing at least one species of Mollicutes. Some oligomers comprise tag regions, target closing reg…
- Who is the assignee on this patent?
- Kaplan Shannon K, Livezey Kristin W, Becker Michael M, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/689. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 15 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).