Method for preparing pyrrolidone
US-2024132925-A1 · Apr 25, 2024 · US
High efficiency FLP site-specific recombination in mammalian cells using an optimized FLP gene
US9206397B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9206397-B2 |
| Application number | US-30741807-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 21, 2007 |
| Priority date | Jul 7, 2006 |
| Publication date | Dec 8, 2015 |
| Grant date | Dec 8, 2015 |
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Abstract
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The present invention provides an optimized FLP site-specific recombinase coding sequence and methods for its use. This genetically engineered FLP gene displays a marked increase in recombination efficiency compared to the native FLP gene and is therefore useful in a wide array of molecular applications.
First claim
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We claim: 1. A nucleic acid molecule comprising an optimized FLP recombinase coding sequence having the sequence of SEQ ID NO:1 that provides enhanced recombinase activity in a cell comprising and expressing the nucleic acid molecule. 2. An expression construct comprising the nucleic acid molecule comprising the optimized FLP recombinase coding sequence of claim 1 . 3. A method of preparing isolated cells comprising an optimized FLP recombin…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N9/00Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9206397B2 cover?
- The present invention provides an optimized FLP site-specific recombinase coding sequence and methods for its use. This genetically engineered FLP gene displays a marked increase in recombination efficiency compared to the native FLP gene and is therefore useful in a wide array of molecular applications.
- Who is the assignee on this patent?
- Raymond Christopher, Soriano Philippe, Hutchinson Fred Cancer Res
- What technology area does this patent fall under?
- Primary CPC classification C12N9/00. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 08 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).