Methods of treatment and screening assays for HIF-1α regulation

Patent metadata

Field	Value
Publication number	US-9205129-B2
Application number	US-201013501066-A
Country	US
Kind code	B2
Filing date	Oct 8, 2010
Priority date	Oct 9, 2009
Publication date	Dec 8, 2015
Grant date	Dec 8, 2015

Abstract

The present invention relates to a method of treating a patient for a condition where blood flow to a tissue or organ is interrupted. This method involves administering to a patient with a hypoxic condition where blood flow to a tissue or organ is interrupted, or at risk thereof, a compound that reduces the rate of HIF-1α inactivation in cells affected by the condition, thereby treating the patient for the condition. Also disclosed is a method of treating a tumor in a patient. In addition, the present invention relates to methods of identifying compounds as candidate drugs for treatment of hypoxic conditions and for treating tumors in a subject.

First claim

What is claimed:

1. A method of identifying compounds as candidate drugs for treatment of hypoxic conditions, said method comprising:
providing a cell expressing Hypoxia-Inducible-Factor-1alpha (HIF-1α) wherein HIF-1α undergoes site-specific cleavage that generates paired HIF-1α fragments;
contacting the cell with a compound to be evaluated;
determining the ability of the compound to inhibit the site-specific cleavage of HIF-1α, wherein said determining involves detecting the…

Assignees

• Halterman Marc
• Univ Rochester

Inventors

• Halterman Marc

Classifications

• A61P35/00

  Human Necessities · mapped topic

• A61P9/00

  Human Necessities · mapped topic

• A61P9/02

  Human Necessities · mapped topic

• A61P9/10

  Human Necessities · mapped topic

• A61K38/1703 Primary

  Human Necessities · mapped topic