Probe set for isothermal one-pot reaction for detecting strains with biologically active biosynthetic pathway and uses thereof
US-2024376553-A1 · Nov 14, 2024 · US
US9200331B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9200331-B2 |
| Application number | US-201013511827-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 17, 2010 |
| Priority date | Dec 3, 2009 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
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The present invention relates to methods for the diagnosis of bacterial vaginosis based on an analysis of a patient sample. For example, patient test samples are analyzed for the presence or absence of one or more lactobacilli and two or more pathogenic organisms. The presence or absence of one or more lactobacilli and two or more pathogenic organisms may be detected using PCR analysis of nucleic acid segments corresponding to each target organism. The quantity of the target organisms can then be used to determine a score which is indicative of a diagnosis of bacterial vaginosis.
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What is claimed is: 1. A method for identifying bacterial vaginosis in a human female subject, the method comprising: (a) obtaining a vaginal swab sample from the subject; (b) measuring levels of Atopobium vaginae, Megasphaera genus and one or more Lactobacilli species selected from the group consisting of Lactobacillus acidophilus, Lactobacillus crispatus , and Lactobacillus jensenii in the sample, wherein the levels of Atopobium vaginae, Megasphaera genus and of one…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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