Comprehensive immunoprofiling of peripheral blood
US-2024177803-A1 · May 30, 2024 · US
Hybridization methods using natural base mismatches
US9200323B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9200323-B2 |
| Application number | US-16228607-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 6, 2007 |
| Priority date | Feb 8, 2006 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
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Abstract
Official abstract text for this publication.
The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.
First claim
Opening claim text (preview).
The invention claimed is: 1. A process for hybridizing an oligonucleotide to a first nucleic acid target, the method comprising the steps of: providing an oligonucleotide comprising a nucleic acid sequence complementary in part to the first target, but comprising at least one artificial mismatch relative to the first target and comprising a nucleic acid sequence complementary in part to a second target, but comprising at least one artificial mismatch and a true mismatch relative…
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Chemistry & Metallurgy · mapped topic
- C12Q1/6881Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9200323B2 cover?
- The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. T…
- Who is the assignee on this patent?
- Gao Huafang, Li Ze, Wang Dong, and 11 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6881. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).