Real-time sequencing methods and systems

Patent metadata

Field	Value
Publication number	US-9200320-B2
Application number	US-201414571892-A
Country	US
Kind code	B2
Filing date	Dec 16, 2014
Priority date	Apr 27, 2009
Publication date	Dec 1, 2015
Grant date	Dec 1, 2015

Abstract

The present invention is generally directed to compositions, methods, and systems for performing single-molecule, real-time analysis of a variety of different biological reactions. The ability to analyze such reactions provides an opportunity to study those reactions as well as to potentially identify factors and/or approaches for impacting such reactions, e.g., to either enhance or inhibit such reactions. In certain preferred embodiments, RNA templates are used in single-molecule real-time sequencing reactions.

First claim

The invention claimed is:

1. A method for identifying a secondary structure of an RNA molecule, the method comprising:
(a) providing the RNA molecule, wherein the RNA molecule comprises a secondary structure important to the biological function of the RNA molecule;
(b) binding a sequencing engine to the RNA molecule to produce a complex;
(c) immobilizing the complex in an optical confinement such that the optical confinement contains only one such complex;
(d) introducing a…

Assignees

• Pacific Biosciences California

Inventors

• Korlach Jonas
• Turner Stephen
• Schadt Eric

Classifications

• C12Q1/6869 Primary

  Chemistry & Metallurgy · mapped topic

• C12Q1/702

  Chemistry & Metallurgy · mapped topic

• C12Q2547/101

  Chemistry & Metallurgy · mapped topic

• C12Q2565/518

  Chemistry & Metallurgy · mapped topic