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Reduced inhibition of one-step RT-PCR
US9200318B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9200318-B2 |
| Application number | US-201213681326-A |
| Country | US |
| Kind code | B2 |
| Filing date | Nov 19, 2012 |
| Priority date | Nov 27, 2007 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
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- Title
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Abstract
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The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothioate oligodeoxythymine or poly(rA)(dT). The mixing forms a mixture that is incubated under conditions sufficient to synthesize a DNA molecule complementary to all or a portion of the RNA template, thereby amplifying the nucleic acid molecule.
First claim
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What is claimed is: 1. A method of reverse transcribing a cDNA molecule from an RNA template, comprising: mixing the RNA template with a composition comprising a reverse transcriptase, a DNA primer, and a phosphorothioate oligodeoxynucleotide, wherein the phosphorothioate oligodeoxynucleotide is present in an amount from 0.01 nM to 25 nM; and incubating the mixture at a temperature sufficient to synthesize a DNA molecule complementary to at least a portion of the RNA template,…
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Chemistry & Metallurgy · mapped topic
- C07K14/195Primary
Chemistry & Metallurgy · mapped topic
- C12Q1/686Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US9200318B2 cover?
- The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothi…
- Who is the assignee on this patent?
- Bio Rad Laboratories, Bio Rad Laboratories
- What technology area does this patent fall under?
- Primary CPC classification C07K14/195. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).