Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
Synthetic siRNA detection method
US9200314B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9200314-B2 |
| Application number | US-201113186941-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 20, 2011 |
| Priority date | Jul 21, 2010 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).
First claim
Opening claim text (preview).
What is claimed is: 1. A method for detecting a synthetic siRNA in which the 3′ end is a dT 1-4 nucleotide sequence, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ end to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by reverse transcription; and (c) detecting the DNA synthe…
Assignees
Inventors
Classifications
- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Patent family
Related publications grouped by family.
External sources
Next steps
Free tools are coming soon. Tell us what you want to track and we'll notify you.
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9200314B2 cover?
- A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added…
- Who is the assignee on this patent?
- Yanagi Satoshi, Kobayashi Eiji, Uemori Takashi, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).