Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
Lysis and reverse transcription for MRNA quantification
US9200313B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9200313-B2 |
| Application number | US-79787810-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 10, 2010 |
| Priority date | May 3, 2007 |
| Publication date | Dec 1, 2015 |
| Grant date | Dec 1, 2015 |
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Abstract
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The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the sample to an extent such that Guanidine Thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time, characterized in that steps a) to c) are done in the same vessel.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for performing a real time polymerase chain reaction (RT-PCR) for amplifying a target RNA comprising the steps of: lysing a biological sample which is supposed to contain the target RNA in a sample vessel with a lysis buffer comprising between 0.05 M and 1 M of Guanidine Thiocyanate and in the presence of NP40 (octyl phenoxylpolyethoxylethanol); diluting the sample to an extent such that the Guanidine Thiocyanate is present during a subsequent re…
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- C12Q1/6806Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12Q1/6851Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US9200313B2 cover?
- The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M Guanidine Thiocyanate, b) diluting the sample to an extent such that Guanidine Thiocyanate is present in a concentration of about 30 to 50 mM, c) r…
- Who is the assignee on this patent?
- Bengtsson Martin, Kubista Michael, Stahlberg Anders, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Dec 01 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).