Smartphone biosensor

Patent metadata

Field	Value
Publication number	US-9185200-B2
Application number	US-201414579804-A
Country	US
Kind code	B2
Filing date	Dec 22, 2014
Priority date	Jan 4, 2013
Publication date	Nov 10, 2015
Grant date	Nov 10, 2015

Abstract

A mobile computing device that includes an image sensor may be used to detect the result of a biomolecular assay. The biomolecular assay may be performed in an optical assay medium that provides an optical output in response to light from a light source, with the optical output indicating result. A wavelength-dispersive element may be used to disperse the optical output into spatially-separated wavelength components. The mobile computing device may be positioned relative to the wavelength-dispersive element such that different wavelength components are received at different locations on the image sensor. With the mobile computing device positioned in this way, the image sensor may be used to obtain one or more images that include the separated wavelength components of the optical output. A wavelength spectrum of the optical output may be determined from the one or more images, and the result may be determined from the wavelength spectrum.

First claim

What is claimed is:

1. A system, comprising:
a mobile computing device, wherein the mobile computing device includes an image sensor, a processor, and a memory that stores program instructions;
an optical instrument, comprising:
a sample chamber for receiving an optical assay medium configured to perform a biomolecular assay, wherein the sample chamber comprises a door that prevents stray light from entering, wherein the optical assay medium comprises at least one fluorophore;
a light source;
an input optical path for directing light from the light source to the optical assay medium in the sample chamber, wherein the input optical path comprises a first multimode fiber;
an output optical path for receiving an optical output from the optical assay medium in the sample chamber, wherein the output optical path comprises a second multimode fiber and a wavelength-dispersive element configured to disperse the optical output into spatially-separated wavelength components; and
a mount for mounting the mobile computing device to the instrument in a fixed position relative to the wavelength-dispersive element, wherein in the fixed position the image sensor of the mobile computing device is optically coupled to the output optical path such that the image sensor receives at least a portion of the dispersed optical output and different wavelength components are received at different locations on the image sensor, wherein the program instructions stored in the memory are executable by the processor to cause the mobile computing device to perform functions, the functions comprising:
using the image sensor to obtain at least one image of the portion of the dispersed optical output; and
determining a wavelength spectrum of the optical output based on the at least one image.

2. The system of claim 1 , wherein the light source is a laser.

3. The system of claim 1 , wherein the optical output comprises a fluorescence emission from the at least one fluorophore excited by light from the light source.

4. The system of claim 3 , wherein an intensity of the fluorescence emission is indicative of a result of the biomolecular assay.

5. The system of claim 3 , wherein a polarization of the fluorescence emission is indicative of a result of the biomolecular assay.

6. The system of claim 1 , wherein the at least one fluorophore comprises a donor fluorophore and an acceptor fluorophore.

7. The system of claim 6 , wherein the optical output comprises a fluorescence emission from the donor fluorophore and/or acceptor fluorophore.

8. The system of claim 1 , wherein the wavelength-dispersive element is a diffraction grating.

9. The system of claim 1 , wherein in the fixed position a user interface of the mobile computing device is accessible.

10. The system of claim 1 , wherein the mobile computing device is a smartphone.

11. The system of claim 1 , wherein the mobile computing device further includes a display and wherein the functions further comprise:
displaying an indication of the wavelength spectrum of the optical output on the display.

12. The system of claim 1 , wherein the mobile computing device further includes a display and wherein the functions further comprise:
determining a result of a biomolecular assay based on the wavelength spectrum of the optical output; and
displaying an indication of the result on the display.

13. The system of claim 1 , wherein the mount is configured to removably mount the mobile computing device to the instrument in a fixed position relative to the wavelength-dispersive element.

Assignees

• Univ Illinois

Inventors

• Cunningham Brian T

Classifications

• H04M1/72412

  using two-way short-range wireless interfaces · CPC title

• H04M1/72409

  by interfacing with external accessories (hands-free H04M1/60) · CPC title

• G01J3/44 Primary

  Raman spectrometry; Scattering spectrometry {; Fluorescence spectrometry} · CPC title

• G01N21/658

  enhancement Raman, e.g. surface plasmons · CPC title

• G01J3/4406

  Fluorescence spectrometry · CPC title