Synthetic production of circular dna vectors
US-2024409975-A1 · Dec 12, 2024 · US
Reusable PCR amplification system and method
US9181571B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9181571-B2 |
| Application number | US-201414488734-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 17, 2014 |
| Priority date | Dec 16, 2005 |
| Publication date | Nov 10, 2015 |
| Grant date | Nov 10, 2015 |
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Abstract
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A DNA amplification device utilizing a polydimethylsiloxane (PDMS) and silicon substrate coated with spin-on glass (SOG) is provided. This PDMS layer is irreversibly bonded to the SOG layer of the silicon substrate using oxygen plasma. The amplification device is an inexpensive, microfluidic device, which can be utilized as a portable thermo-cycler to perform PCR amplification of DNA in the field.
First claim
Opening claim text (preview).
What is claimed is: 1. A method for amplifying a DNA sample on an amplification chip for DNA amplification, the method comprising: providing the DNA sample having a concentration of 1 picogram per microliter or less; denaturing the DNA sample on the amplification chip, wherein the inner surfaces of the amplification chip consist of a polydimethylsiloxane layer bonded to a spin-on glass layer; associating the DNA sample with primers on the amplification chip; and amplifying t…
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Operations & Transport · mapped topic
Operations & Transport · mapped topic
Operations & Transport · mapped topic
Operations & Transport · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US9181571B2 cover?
- A DNA amplification device utilizing a polydimethylsiloxane (PDMS) and silicon substrate coated with spin-on glass (SOG) is provided. This PDMS layer is irreversibly bonded to the SOG layer of the silicon substrate using oxygen plasma. The amplification device is an inexpensive, microfluidic device, which can be utilized as a portable thermo-cycler to perform PCR amplification of DNA in the field.
- Who is the assignee on this patent?
- Univ Missouri
- What technology area does this patent fall under?
- Primary CPC classification C12P19/34. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 10 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).