Water-dispersable nanoparticles

Patent metadata

Field	Value
Publication number	US-9174187-B2
Application number	US-200913057504-A
Country	US
Kind code	B2
Filing date	Aug 6, 2009
Priority date	Aug 6, 2008
Publication date	Nov 3, 2015
Grant date	Nov 3, 2015

Abstract

Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

First claim

What is claimed:

1. A population of water-dispersible nanoparticles, comprising:
a plurality of nanoparticles,
wherein each nanoparticle includes a nanocrystal core including a first semiconductor material, a shell including a second semiconductor material, and an outer layer that imparts hydrophilic properties to the nanoparticle,
wherein the outer layer is comprised of a plurality of hydrophilic peptide ligands and a plurality of phosphonic acid ligands each with at least one linking group for attachment to a surface of the shell,
wherein the ratio of the plurality of hydrophilic ligands to the plurality of phosphonic acid ligands is at least about 0.1:1.

2. The population of water-dispersible nanoparticles of claim 1 , wherein the phosphonic acid ligand is tetradecylphosphonic acid (TDPA) or octylphosphonic acid (OPA).

3. The population of water-dispersible nanoparticles of claim 1 , wherein the plurality of hydrophilic ligands are cross-linked to each other.

4. The population of water-dispersible nanoparticles of claim 1 , wherein the nanocrystal core is CdSe and the shell is ZnS.

5. A population of water-dispersible nanoparticles, comprising:
a plurality of nanoparticles, wherein each nanoparticle includes a nanocrystal core including a first semiconductor material, a shell including a second semiconductor material, and an outer layer that imparts hydrophilic properties to the nanoparticle,
wherein the outer layer is comprised of a plurality of tridentate thiol ligands and a plurality of phosphonic acid ligands each with at least one linking group for attachment to a surface of the shell,
wherein the ratio of the plurality of tridentate thiol ligands to the plurality of phosphonic acid ligands is at least about 0.5:1, wherein the tridentate thiol ligand is a compound of Formula I, II, III, IV, V, or VI:

wherein R 1 , R 2 , and R 3 , when taken alone, are independently H, halo, hydroxyl, (—(C═O)—C 1 -C 22 , —(C═O)CF 3 ,) alkanoyl, C 1 -C 22 alkyl, C 1 -C 22 heteroalkyl, ((CO)OC 1 -C 22 ) alkylcarbonato, or (—(CO)NH(C 1 -C 20 ) or —(CO)N(C 1 -C 20 ) 2 ) alkylcarbamoyl;
R 4 , and R 5 , when taken alone, are independently H, C 1 -C 20 alkyl, or C 6 -C 18 aryl; and
R 6 is H or a polyethylene glycol moiety.

6. The population of water-dispersible nanoparticles of claim 5 , wherein R 1 , R 2 , and R 3 are different.

7. The population of water-dispersible nanoparticles of claim 5 , wherein R 1 , R 2 , and R 3 are the same.

8. The population of water-dispersible nanoparticles of claim 5 , wherein R 4 and R 5 are different.

9. The population of water-dispersible nanoparticles of claim 5 , wherein R 4 and R 5 are the same.

10. The population of water-dispersible nanoparticles of claim 5 , wherein the polyethylene glycol moiety is a compound of Formula VII:

wherein R 7 is NH 2 , N 3 , NHBoc, NHFmoc, NHCbz, COOH, COOt-Bu, COOMe, iodoaryl, hydroxyl, alkyne, boronic acid, allylic alcohol carbonate, NHBiotin, CO)NHNHBoc, (CO)NHNHFmoc, or OMe,
wherein n is an integer from 1 to 100.

11. The population of water-dispersible nanoparticles of claim 5 , wherein the phosphonic acid ligand is one of TDPA or OPA.

12. The population of water-dispersible nanoparticles of claim 5 , wherein the nanocrystal core is CdSe and the shell is ZnS.

Assignees

• Tulsky Eric
• Haley Kari
• Naasani Imad
• Mauro John
• Rozhkov Roman
• Treadway Joseph A
• Life Technologies Corp

Inventors

• Tulsky Eric
• Haley Kari
• Naasani Imad
• Mauro John
• Rozhkov Roman
• Treadway Joseph A

Classifications

• B22F1/0545

  Dispersions or suspensions of nanosized particles · CPC title

• B22F1/102

  Metallic powder coated with organic material · CPC title

• B01J13/14 Primary

  Polymerisation; cross-linking · CPC title

• Y10S977/81

  Of specified metal or metal alloy composition · CPC title

• Y10S977/834

  Optical properties of nanomaterial, e.g. specified transparency, opacity, or index of refraction · CPC title