Genetically modified bacillus subtilis strain and use as a live delivery and production system
US-2024390433-A1 · Nov 28, 2024 · US
US9150868B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9150868-B2 |
| Application number | US-200913126154-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 30, 2009 |
| Priority date | Nov 5, 2008 |
| Publication date | Oct 6, 2015 |
| Grant date | Oct 6, 2015 |
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Disclosed is an Escherichia coli producing 2-deoxy-scyllo-inosose (DOI), which, from a sucrose non-PTS gene group, has at least a sucrose hydrolase (CscA)-encoding gene and which is provided with a DOI production system or has an enhanced DOI production system. The Escherichia coli preferably further includes a system to enhance sugar uptake capacity. There is also disclosed a method of producing DOI from a plant-derived raw material containing sucrose by using the Escherichia coli.
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The invention claimed is: 1. A 2-deoxy-scyllo-inosose (DOI)-producing Escherichia coli , which is provided with a DOI production system or has an enhanced DOI production system by introducing a DOI synthase (BtrC)-encoding gene derived from Bacillus circulans into a host, wherein a phosphoglucose isomerase (Pgi)-encoding gene and a glucose-6-phosphate-1-dehydrogenase (Zwf)-encoding gene of the host are disrupted, and only a sucrose hydrolase (CscA)-encoding gene derived from E…
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