Mesenchymal stem cells (MSC) expansion methods and materials
US-9220810-B2 · Dec 29, 2015 · US
Methods of proliferating stem cells
US9145546B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9145546-B2 |
| Application number | US-32946008-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 5, 2008 |
| Priority date | Feb 11, 2005 |
| Publication date | Sep 29, 2015 |
| Grant date | Sep 29, 2015 |
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Abstract
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The invention relates to methods of proliferating stem cells. More particularly, the invention relates to the use of glycosaminoglycans or proteoglycans to promote the growth of stem cells in ex vivo culture, while preserving their multipotentiality.
First claim
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What is claimed is: 1. An in vitro culture of mesenchymal stem cells, the cell culture comprising: (i) an isolated CD34+ fraction of multipotent human mesenchymal stem cells (hMSCs) expressing STRO-1 and having an ability to generate cartilage, bone, muscle, tendon, ligament and fat, and (ii) a cell culture medium comprising isolated heparin sulfate 2 (HS2) at a concentration that ranges between about 6.4 ng/ml to 20 μg/ml culture media, wherein hMSCs cultured in contact with HS2 for a given period of time undergo more population doubling than hMSCs without HS2. 2. The in vitro culture of mesenchymal stem cells according to claim 1 wherein the mesenchymal stem cells are adult mesenchymal stem cells. 3. A method of regenerating tissue in a patient in need of such treatment, the method comprising administering to the patient a therapeutic number of mesenchymal stem cells obtained from the in vitro culture according to claim 1 .
Assignees
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Classifications
- C12N5/0663Primary
Bone marrow mesenchymal stem cells (BM-MSC) · CPC title
Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00 · CPC title
Heparin · CPC title
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- What does patent US9145546B2 cover?
- The invention relates to methods of proliferating stem cells. More particularly, the invention relates to the use of glycosaminoglycans or proteoglycans to promote the growth of stem cells in ex vivo culture, while preserving their multipotentiality.
- Who is the assignee on this patent?
- Nurcombe Victor, Cool Simon, Agency Science Tech & Res
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0663. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Sep 29 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).