Recombinant expression vector applicable to rapid screening for recombinant strain and application
US-12037632-B2 · Jul 16, 2024 · US
US9133448B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9133448-B2 |
| Application number | US-201113578223-A |
| Country | US |
| Kind code | B2 |
| Filing date | Feb 11, 2011 |
| Priority date | Feb 11, 2010 |
| Publication date | Sep 15, 2015 |
| Grant date | Sep 15, 2015 |
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The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.
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The invention claimed is: 1. A variant polypeptide having at least 95% sequence identity with the sequence set out in SEQ ID NO: 2 or the mature form thereof, wherein said variant polypeptide or mature form thereof has more than one substitution or insertion relative to SEQ ID NO: 2 and wherein said variant polypeptide is capable of hydrolyzing cellulose. 2. A polynucleotide encoding the variant polypeptide according to claim 1 or the reverse complement there…
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