Diagnosing cancer using genomic sequencing

Patent metadata

Field	Value
Publication number	US-9121069-B2
Application number	US-201313937162-A
Country	US
Kind code	B2
Filing date	Jul 8, 2013
Priority date	Jul 23, 2007
Publication date	Sep 1, 2015
Grant date	Sep 1, 2015

Abstract

Methods, systems, and apparatus determine whether a first chromosomal region exhibits a deletion or an amplification associated with cancer in a sample from a subject (e.g., where the sample includes a mixture of cell-free DNA from tumor cells and non-malignant cells. Nucleic acid molecules of the biological sample are sequenced. Respective amounts of a clinically-relevant chromosomal region and of background chromosomal region(s) are determined from results of the sequencing. A parameter derived from these amounts (e.g. a ratio) is compared to one or more cutoff values, thereby determining a classification of whether first chromosomal region exhibits a deletion or an amplification associated with cancer.

First claim

What is claimed is:

1. A method of analyzing a biological sample of a human for deletions or amplifications in one or more chromosomal regions associated with cancer, the biological sample including cell-free nucleic acid molecules originating from non-malignant cells and potentially from tumor cells associated with cancer, the method comprising:
performing a random sequencing of nucleic acid molecules from biological sample by:
attaching adaptors to at least one end of the nucleic acid molecules; and
sequencing, using the adaptors, the nucleic acid molecules to obtain sequences, including at least 120,000 sequences;

receiving, at a computer system, the sequences obtained from the random sequencing of nucleic acid molecules contained in the biological sample;
aligning, by the computer system, at least a portion of the sequences to a human genome;
determining, by the computer system, a first amount of sequences identified as aligning to a first chromosomal region that is part of a first chromosome, wherein a deletion or an amplification in the first chromosomal region is associated with cancer;
determining, by the computer system, a second amount of sequences identified as aligning to one or more second chromosomal regions;
determining a first parameter from the first amount and the second amount, wherein the first parameter represents a relative amount between the first and second amounts; and
comparing the first parameter to one or more cutoff values to determine a classification of whether the first chromosomal region exhibits a deletion or an amplification associated with cancer in the human.

2. The method of claim 1 , wherein the classification includes whether the first chromosomal region is amplified, and wherein a classification of amplification is made when the first parameter is greater than a first cutoff value of the one or more cutoff values.

3. The method of claim 1 , wherein the classification includes whether the first chromosomal region has a deletion, and wherein a classification of deletion is made when the first parameter is less than a first cutoff value of the one or more cutoff values.

4. The method of claim 1 , further comprising:
determining a third amount of sequences identified as aligning to a third chromosomal region, wherein an aberration in the third chromosomal region is associated with cancer;
determining a second parameter for the third chromosomal region, wherein the second parameter represents a relative amount between the third and second amounts;
comparing the second parameter to one or more cutoff values to determine a classification of whether the third chromosomal region exhibits a deletion and/or an amplification associated with cancer; and
using the classifications of the first and third chromosomal regions to determine whether the human has cancer.

5. The method of claim 1 , wherein the biological sample is blood, plasma, serum, urine or saliva.

6. The method of claim 1 , wherein the first parameter is calculated from a ratio that includes the first amount.

7. The method of claim 6 , wherein the ratio provides a percentage of the sequences identified as aligning to the first chromosomal region among the sequences.

8. The method of claim 6 , wherein the ratio is obtained from any one or more of a fractional count of the number of sequenced tags, a fractional number of sequenced nucleotides, and a fractional length of accumulated sequences.

9. The method of claim 6 , wherein the sequences that are used to determine the first amount are selected to be less than a specified number of bases.

10. The method of claim 9 , wherein the specified number of bases is 300 bases, 200 bases, or 100 bases.

11. The method of claim 1 , wherein the nucleic acid molecules of the biological sample have been enriched for sequences originating from the first chromosomal region.

12. The method of claim 1 , wherein the nucleic acid molecules of the biological sample have been enriched for sequences less than 300 bases or enriched for sequences less than 200 bases.

13. The method of claim 1 , wherein the nucleic acid molecules of the biological sample have been amplified using a polymerase chain reaction.

14. The method of claim 1 , wherein the aligned portion represents at least a pre-determined fraction of the human genome.

15. The method of claim 1 , wherein the fraction represents at least 0.1% of the human genome or represents at least 0.5% of the human genome.

16. The method of claim 1 , wherein at least one of the cutoff values is derived from a percentage of tumor cells in the biological sample.

17. The method of claim 1 , wherein at least one of the cutoff values is a reference value established in a normal biological sample.

18. The method of claim 17 , wherein the at least one cutoff value is determined from a mean of the value of the first parameter in a plurality of normal biological samples.

19. The method of claim 1 , further comprising:
identifying an amount of tumor DNA in the biological sample; and
calculating a number N of sequences to be analyzed based on a desired accuracy.

20. The method of claim 1 , wherein the sequences identified as aligning to the first chromosomal region uniquely align to the first chromosomal region.

21. The method of claim 1 , wherein the sequences identified as aligning to the one or more of the second chromosomal regions uniquely align to only one of the second chromosomal regions.

22. The method of claim 1 , wherein the human genome is repeat-masked.

23. The method of claim 1 , wherein the first amount is determined based on a pool of sequenced tags that align to a plurality of positions of the first chromosomal region.

24. The method of claim 23 , wherein which part of the human genome that the sequenced tags are aligned is not pre-determined.

25. The method of claim 24 , wherein the part of the human genome corresponding to the sequenced tags aligned to the first chromosomal region is not pre-determined.

26. The method of claim 6 , wherein the first parameter is a fractional representation of sequences identified as aligning to the first chromosomal region, the fractional representation being a measure of a proportion of nucleic acid molecules in the biological sample that are from the first chromosomal region.

27. The method of claim 26 , wherein the one or more cutoff values take into account a size of the first chromosomal region relative to a size of the one or more second chromosomal regions.

28. The method of claim 1 , wherein the sequencing includes paired-end sequencing, and wherein aligning each sequence includes aligning paired-end sequences.

29. The method of claim 1 , wherein the cell-free nucleic acid molecules are DNA fragments.

Assignees

• Univ Hong Kong Chinese

Inventors

• Lo Yuk-Ming Dennis
• Chiu Rossa Wai Kwun
• Chan Kwan Chee

Classifications

• G16B30/00

  ICT specially adapted for sequence analysis involving nucleotides or amino acids · CPC title

• G16B20/00

  ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations · CPC title

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6809

  Methods for determination or identification of nucleic acids involving differential detection · CPC title

• C12Q1/6827

  for detection of mutation or polymorphism · CPC title