Methods for cleaning and sterilization of implant tissue ex vivo with shock waves

Patent metadata

Field	Value
Publication number	US-9119888-B2
Application number	US-201414228897-A
Country	US
Kind code	B2
Filing date	Mar 28, 2014
Priority date	Sep 17, 2009
Publication date	Sep 1, 2015
Grant date	Sep 1, 2015

Abstract

This invention includes methods for cleaning and sterilizing tissue harvested for implant with shock waves by submerging the harvested tissue in a volume of fluid in a holding vessel including a shock wave applicator with a focal volume in the volume of fluid, and applying a pressure shock wave pulses to the harvested tissue from the shock wave applicator in sufficient amounts to eliminate biological contaminants in the harvested tissue.

First claim

What is claimed is:

1. A method comprising;
providing a volume of fluid in a holding vessel, wherein the holding vessel includes a shock wave applicator configured to have a focal volume disposed in the volume of fluid;
submerging at least a portion of a harvested tissue including biological contaminants into the volume of fluid; and
applying a plurality of pressure shock wave pulses, by the shock wave applicator, to the at least a portion of harvested tissue in the volume of fluid in sufficient amounts to damage at least some of biological contaminants in the harvested tissue.

2. The method of claim 1 , wherein the harvested tissue is a soft tissue.

3. The method of claim 2 , wherein the soft tissue has a volume similar to the focal volume of the shock wave applicator, and the soft tissue is submerged within the focal volume of the shock wave applicator.

4. The method of claim 2 , further comprising;
wherein the soft tissue has a volume larger than the focal volume of the shock wave applicator; and
moving the shock wave applicator over at least a portion of a surface of the soft tissue during applying the plurality of pressure shock wave pulses.

5. The method of claim 4 , wherein the moving of the shock wave generator is performed manually.

6. The method of claim 4 , wherein the moving of the shock wave applicator is controlled by a controller actuated by a computer.

7. The method of claim 2 , wherein the shock wave applicator applies between 50 and 500 pressure shock wave pulses per cm 3 of soft tissue at an energy flux density between 0.10 and 0.40 mJ/mm 2 .

8. The method of claim 2 , wherein the soft tissue is at least one of a portion of skin, at least a portion of a tendon, at least a portion of a ligament, and at least a portion of a collagen matrix.

9. The method of claim 1 , wherein the harvested tissue is a hard tissue.

10. The method of claim 9 , wherein the hard tissue has a volume similar to the focal volume of the shock wave applicator, and the hard tissue is submerged within the focal volume of the shock wave applicator.

11. The method of claim 9 , further comprising;
wherein the hard tissue has a volume larger than the focal volume of the shock wave applicator; and
moving the shock wave applicator over at least a portion of a surface of the hard tissue during applying the plurality of pressure shock wave pulses.

12. The method of claim 11 , wherein the moving of the shock wave generator is performed manually.

13. The method of claim 11 , wherein the moving of the shock wave applicator is controlled by a controller actuated by a computer.

14. The method of claim 9 , wherein the shock wave applicator applies more than 1,000 pressure shock wave pulses per cm 3 of hard tissue at an energy flux density between 0.40 and 0.90 mJ/mm 2 .

15. The method of claim 9 , wherein the hard tissue is at least one of a at least a portion of bone, at least a portion of a cartilage, and at least a portion of a bone matrix.

16. The method of claim 1 , wherein the volume of fluid is a volume of saline fluid.

17. The method of claim 1 , wherein the biological contaminants are one or more of bacteria, viruses, and microorganisms.

18. The method of claim 1 , further comprising submerging the harvested tissue in direct contact with the fluid in the holding vessel.

19. The method of claim 1 , further comprising submerging the harvested tissue in an enclosure in the holding vessel.

Assignees

• Sanuwave Inc
• Royal Group Inc

Inventors

• Cioanta Iulian
• Spenninck Yannick

Classifications

• A23B4/015

  Preserving by irradiation or electric treatment without heating effect · CPC title

• A61L2202/14

  Means for controlling sterilisation processes, data processing, presentation and storage means, e.g. sensors, controllers, programs · CPC title

• A61L2202/11

  Apparatus for generating biocidal substances, e.g. vaporisers, UV lamps · CPC title

• B06B3/04

  involving focusing or reflecting · CPC title

• A61L2/02 Primary

  using physical processes · CPC title