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Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
US9115409B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9115409-B2 |
| Application number | US-201313794199-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 11, 2013 |
| Priority date | Sep 27, 2007 |
| Publication date | Aug 25, 2015 |
| Grant date | Aug 25, 2015 |
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Abstract
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Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
First claim
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What is claimed is: 1. A host cell comprising an endogenous genome and an episomal reporter construct, the episomal reporter construct comprising multiple target sites for at least one nuclease comprising a TAL-effector domain, the target sites flanked by sequences encoding a disabled reporter gene, wherein the endogenous genome comprises the target sites and further wherein the sequences encoding the disabled reporter comprise identical repeated sequences flanking the target seque…
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- C12Q1/6897Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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Frequently asked questions
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- What does patent US9115409B2 cover?
- Disclosed herein are methods and compositions for rapidly identifying active nucleases and cells having nuclease-mediated genomic modifications.
- Who is the assignee on this patent?
- Sangamo Biosciences Inc
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/6897. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 25 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).