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Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use
US9115397B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9115397-B2 |
| Application number | US-201414488751-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 17, 2014 |
| Priority date | May 11, 2005 |
| Publication date | Aug 25, 2015 |
| Grant date | Aug 25, 2015 |
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Abstract
Official abstract text for this publication.
Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA.
First claim
Opening claim text (preview).
What is claimed is: 1. A method of detecting the presence or absence of double stranded DNA in a sample, the method comprising: providing a sample suspected of containing double stranded DNA; contacting the sample with a chemical compound to prepare a test sample; illuminating the test sample with energy; and detecting emission of energy from the test sample; wherein the chemical compound has the structure:…
Assignees
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Classifications
- C07D215/10Primary
Chemistry & Metallurgy · mapped topic
- C12Q1/6876Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9115397B2 cover?
- Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively o…
- Who is the assignee on this patent?
- Life Technologies Corp
- What technology area does this patent fall under?
- Primary CPC classification C07D215/10. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 25 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).