METHODS AND SYSTEMS FOR FUNCTIONAL MATURATION OF iPSC AND ESC DERIVED CARDIOMYOCYTES
US-2024076619-A1 · Mar 7, 2024 · US
Method for purifying cardiomyocytes or programmed cardiomyocytes derived from stem cells or fetuses
US9115342B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9115342-B2 |
| Application number | US-16268407-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 31, 2007 |
| Priority date | Jan 31, 2006 |
| Publication date | Aug 25, 2015 |
| Grant date | Aug 25, 2015 |
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Abstract
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An object of the present invention is to develop a method for purify cardiomyocytes at a high degree of purification and at a high yield from a cell mixture comprising cardiomyocytes derived from fetuses and stem cells using various features which have not been previously expected to be used for purification of cardiomyocytes or which are newly found, wherein said method is carried out without undergoing any genetic modification or without adding any special proteins or biologically active agents.
First claim
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The invention claimed is: 1. A method of selecting cardiomyocytes and/or Brachyury+/Nkx2.5-cells capable of differentiating into cardiomyocytes from a cell mixture containing cardiomyocytes, Brachyury+/Nkx2.5-cells, and non-cardiomyocyte cells comprising: Culturing the cell mixture in a culture medium under the following conditions: (i) a sugar-free condition or a condition wherein sugar content is 111.20 μM or less; and (ii) one or more conditions selected from the group cons…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12N5/0657Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US9115342B2 cover?
- An object of the present invention is to develop a method for purify cardiomyocytes at a high degree of purification and at a high yield from a cell mixture comprising cardiomyocytes derived from fetuses and stem cells using various features which have not been previously expected to be used for purification of cardiomyocytes or which are newly found, wherein said method is carried out without …
- Who is the assignee on this patent?
- Hattori Fumiyuki, Fukuda Keiichi, Daiichi Sankyo Company, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N5/0657. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 25 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).