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Nanometer-scale optical imaging by the modulation tracking (MT) method
US9110282B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9110282-B2 |
| Application number | US-201213435387-A |
| Country | US |
| Kind code | B2 |
| Filing date | Mar 30, 2012 |
| Priority date | Mar 30, 2011 |
| Publication date | Aug 18, 2015 |
| Grant date | Aug 18, 2015 |
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Abstract
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An optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved three dimensional images of very small and moving features in live cells and in a matter of seconds. Images of microvilli in live cultured opossum kidney cells expressing NaPi co-transporter proteins with different GFP constructs and images of cell protrusions in a collagen matrix are produced with a resolution of about 20 nm. Along cell protrusions in three dimensional cellular adhesions could be identified to the extracellular matrix.
First claim
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We claim: 1. A method of optical imaging for producing a nanometer-resolved three dimensional image of a feature of an object comprising: moving a laser spot around a surface of the feature in a circular orbit; oscillating a radial position of the laser spot perpendicularly with respect to the surface of the feature as the laser spot is being moved in the circular orbit around the surface of the feature, wherein the laser spot has a cross section characterized by a point spread…
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Physics · mapped topic
Operations & Transport · mapped topic
Physics · mapped topic
Physics · mapped topic
- G02B21/0012Primary
Physics · mapped topic
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- What does patent US9110282B2 cover?
- An optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved three dimensional images of very small and moving features in live cells and in a matter of seconds. Images of microvilli in live cultured opossum kidney cells expressing NaPi co-transporter proteins with di…
- Who is the assignee on this patent?
- Lanzano Luca, Digman Michelle, Gratton Enrico, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification G02B21/0012. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Aug 18 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).