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Engineered transposon for facile construction of a random protein domain insertion library
US9109225B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9109225-B2 |
| Application number | US-201314025945-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 13, 2013 |
| Priority date | Sep 14, 2012 |
| Publication date | Aug 18, 2015 |
| Grant date | Aug 18, 2015 |
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Abstract
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Methods for facile construction of a random domain insertion library (1) with optimal control of composition and length of inter-domain linker residues and (2) mediated by sticky-end ligation between host and guest DNA fragments. To develop such a method, we engineered a Mu transposon. The method exploits transposition of the engineered Mu transposon, which, upon removal, allows for sticky-end ligation between host and guest DNA fragments. We used a gene coding for xylanase from bacillus circulans (BCX) as a guest DNA sequence and the plasmid PUC19 containing lacZα as the target for insertion (i.e., a host DNA sequence). Results demonstrate that the method enables facile construction of a random domain insertion library with optimal control of composition and length of inter-domain linker residues.
First claim
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What is claimed is: 1. A method for facile construction of a random domain insertion library comprising the steps of: providing an engineered transposon; randomly transposing the engineered transposon into a host DNA sequence, using plasmid PUC19 containing lacZα as the host DNA sequence; employing sticky-end ligation between the host DNA sequence and a guest DNA sequence, using a gene coding for xylanase from bacillus circulans (BCX) as the guest DNA sequence; and removin…
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- C12N15/1082Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9109225B2 cover?
- Methods for facile construction of a random domain insertion library (1) with optimal control of composition and length of inter-domain linker residues and (2) mediated by sticky-end ligation between host and guest DNA fragments. To develop such a method, we engineered a Mu transposon. The method exploits transposition of the engineered Mu transposon, which, upon removal, allows for sticky-end …
- Who is the assignee on this patent?
- Politechnic Inst Univ New York, Univ New York
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1082. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 18 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).