Device for harvesting bacterial colony and method therefor

Patent metadata

Field	Value
Publication number	US-9109194-B2
Application number	US-201013505803-A
Country	US
Kind code	B2
Filing date	Nov 5, 2010
Priority date	Nov 5, 2009
Publication date	Aug 18, 2015
Grant date	Aug 18, 2015

Abstract

When multiple kinds of bacterial colonies are present in a petri dish and, for example, a drug tolerance is to be measured, harvesting of mixed colonies of different types of bacteria makes it impossible to accurately determine the drug tolerance. Also, it is required to improve the throughput of a device for harvesting a bacterial colony. From images illuminated from multiple directions, isolating bacterial colonies are automatically extracted. Next, the image feature amounts are calculated from the multiple images that are illuminated from multiple directions and colonies are grouped depending on the feature amounts. Then, bacterial colonies to be harvested are determined based on the results of the grouping.

First claim

The invention claimed is:

1. A device for harvesting a bacterial colony, comprising:
an upper illumination unit comprising a high-angle illumination unit and a low-angle illumination unit, the high-angle illumination unit and the low angle illumination unit both configured to illuminate a bacterial culture on a culture medium received in a first vessel, which is optically transparent, from above;
a transmitted illumination unit configured to illuminate the bacterial culture by transmitting the optically transparent vessel and the culture medium with illumination light;
an imaging unit configured to sequentially acquire an image the bacterial culture illuminated by high-angle illumination unit of the upper illumination unit, an image of the bacterial culture illuminated by the low-angle illumination of the upper illumination unit, wherein said low-angle illumination image is not produced from directly reflected light and includes color information, and an image of the bacterial culture illuminated by the transmitted illumination unit;
an image processing unit configured to extract an image of a bacterial colony within the bacterial culture to be harvested by processing the image of the bacterial culture illuminated by the high-angle illumination unit of the upper illumination unit, the image of the bacterial culture illuminated by the low-angle illumination unit, and the image of the bacterial culture illuminated by the transmitted illumination unit;
a harvesting unit configured to harvest from the culture medium the bacterial colony corresponding to the image extracted by the image processing unit move the harvested bacterial colony to a second vessel; and
a harvested bacteria number calculating unit configured to calculate the number of harvested bacteria required to prepare a bacterial liquid having a predetermined concentration by using thickness information of the bacterial colony of several steps prepared in advance according to the size of an area of the bacterial colony from the image of the bacterial colony extracted with the image processing unit.

2. The device for harvesting a bacterial colony according to claim 1 , wherein the thickness information of the bacterial colony is capable of being set by a user.

3. A method for harvesting a bacterial colony, comprising:
illuminating from a high angle and a low angle a bacterial culture on a culture medium received in a first vessel, which is optically transparent, from above and imaging the bacterial culture when illuminated from the high angle to acquire above high angle illumination image of the bacterial culture and imaging the bacterial culture when illuminated from the low angle to acquire an above low angle illumination image of the bacterial culture, wherein said low-angle illumination image is not produced from directly reflected light and includes color information;
illuminating the bacterial colony by transmitting the optically transparent vessel and the culture medium with illumination light from below the first vessel and imaging the bacterial culture to acquire a transmitted light illumination image of the bacterial culture;
extracting an image of a bacterial colony within the bacterial culture to be harvested by processing the high angle illumination image, the low angle illumination image and the transmitted light illumination image; and
harvesting the bacterial colony corresponding to the extracted image from the culture medium and moving the harvested bacterial colony to a second vessel,
wherein the number of harvested bacteria required to prepare a bacterial liquid having a predetermined concentration is calculated by using thickness information of the bacterial colony of several steps prepared in advance according to the size of an area of the bacterial colony from the image of the bacterial colony of the upper imaged image.

4. The method for harvesting a bacterial colony according to claim 3 , wherein the thickness information of the bacterial colony is capable of being set by a user.

Assignees

• Honda Toshifumi
• Fujita Hiroko
• Maeshima Muneo
• Maekawa Akira
• Ishida Yoshiko
• Urano Yuta
• Murakami Shinya
• Hitachi High Tech Corp

Inventors

• Honda Toshifumi
• Fujita Hiroko
• Maeshima Muneo
• Maekawa Akira
• Ishida Yoshiko
• Urano Yuta
• Murakami Shinya

Classifications

• C12M33/00 Primary

  Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus (chemical or physical laboratory apparatus in general B01L, devices for taking cell samples A61B10/0045, withdrawing or distributing predetermined quantities of fluid B01L99/00) · CPC title

• C12M41/36

  of biomass, e.g. colony counters or by turbidity measurements (electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404) · CPC title

• C12M33/02 Primary

  by impregnation, e.g. using swabs or loops (fluid transport using swabs B01L3/5029) · CPC title