Apparatus and method for cell kill confirmation
US-12168779-B2 · Dec 17, 2024 · US
US9104030B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9104030-B2 |
| Application number | US-201414297082-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jun 5, 2014 |
| Priority date | Jan 17, 2012 |
| Publication date | Aug 11, 2015 |
| Grant date | Aug 11, 2015 |
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An illumination system is disclosed for providing dual-excitation wavelength illumination for non-linear optical microscopy and micro-spectroscopy. The illumination system includes a laser system, an optical splitting means, a frequency shifting system, and a picosecond amplifier system. The laser system includes a laser for providing a first train of pulses at a center optical frequency ω 1 . The optical splitting means divides the first train of pulses at the center optical frequency ω 1 into two trains of pulses. The frequency shifting system shifts the optical frequency of one of the two trains of pulses to provide a frequency shifted train of pulses. The picosecond amplifier system amplifies the frequency shifted train of pulses to provide an amplified frequency-shifted train of pulses having a pulse duration of at least 0.5 picoseconds.
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What is claimed is: 1. An illumination system for providing dual-excitation wavelength illumination for non-linear optical microscopy and micro-spectroscopy, said illumination system comprising: a laser system including a laser for providing a first train of pulses at a center optical frequency ω 1 ; an optical splitting means for dividing the first train of pulses at the center optical frequency ω 1 into a first split train of pulses and a second split train of pulses; a fre…
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