Volumetric Imaging
US-2024418652-A1 · Dec 19, 2024 · US
US9103718B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9103718-B2 |
| Application number | US-201313888868-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 7, 2013 |
| Priority date | Nov 25, 2010 |
| Publication date | Aug 11, 2015 |
| Grant date | Aug 11, 2015 |
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The inventive technique of detecting and analyzing light from a light-emitting particle in accordance with the scanning molecule counting method using an optical measurement with a confocal microscope or a multiphoton microscope is characterized by detecting intensities of components of two or more wavelength bands of light from a light detection region of an optical system with moving the position of the light detection region in a sample solution by changing the optical path of the optical system of the microscope; detecting individually signals of the light from each light-emitting particle in the intensities of the components of the two or more wavelength bands of the detected light; and identifying a kind of light-emitting particle based on the intensities of the components of the two or more wavelength bands of the signals of the light of the detected light-emitting particle.
Opening claim text (preview).
The invention claimed is: 1. An optical analysis device which detects and analyzes light from a light-emitting particle dispersed and moving at random in a sample solution using an optical system of a confocal microscope or a multiphoton microscope, the device comprising: a light detection region moving portion which moves a position of a light detection region of the optical system in the sample solution by changing an optical path of the optical system; a light detecting porti…
Physics · mapped topic
Physics · mapped topic
Physics · mapped topic
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