Bacterial colicin-immunity protein protein purification system
US-2024417426-A1 · Dec 19, 2024 · US
Process for production of protein
US9102971B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9102971-B2 |
| Application number | US-201113326873-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 15, 2011 |
| Priority date | Dec 15, 2010 |
| Publication date | Aug 11, 2015 |
| Grant date | Aug 11, 2015 |
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Abstract
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The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; and a method for producing the protein of interest comprising suspension-culturing a suspension mammalian cell which produces the protein of interest; and an a suspension mammalian cell which expresses the protein of interest.
First claim
Opening claim text (preview).
What is claimed is: 1. A method selected from the group consisting of (1)-(3): (1) a method for producing a protein of interest, comprising introducing at least one expression vector which comprises a gene fragment containing a DNA sequence encoding the protein of interest, and which also comprises a pair of transposon sequences derived from a pair of Tol1 transposons or from a pair of Tol2 transposons at both terminals of the gene fragment, into a suspension mammalian cell capabl…
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Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
- C12P21/02Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9102971B2 cover?
- The present invention relates to a method for integrating a gene fragment inserted between a pair of transposon sequences into a chromosome of a mammalian cell, comprising introducing at least one expression vector which comprises a gene fragment comprising a DNA encoding a protein of interest and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspe…
- Who is the assignee on this patent?
- Kurokawa Megumi, Yamaguchi Keina, Ogawa Risa, and 5 more
- What technology area does this patent fall under?
- Primary CPC classification C12P21/02. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 11 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).