Synthetic production of circular dna vectors
US-2024409975-A1 · Dec 12, 2024 · US
US9102943B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9102943-B2 |
| Application number | US-201113823805-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 4, 2011 |
| Priority date | Oct 5, 2010 |
| Publication date | Aug 11, 2015 |
| Grant date | Aug 11, 2015 |
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The present invention provides a method for producing a virus vector, which comprises a step wherein cells that are capable of producing a virus vector are cultured in a culture medium that contains, as active components, a retinoic acid and a histone deacetylase inhibiting substance; and a culture medium for the production of a virus vector, which is characterized by containing, as active components, a retinoic acid and a histone deacetylase inhibiting substance.
Opening claim text (preview).
The invention claimed is: 1. A method of producing a retrovirus particle, which comprises a step of culturing a producer cell line which produces the retrovirus particle and producing the retrovirus particle in a culture medium containing retinoic acid and a histone deacetylase inhibitor as active ingredients and a step of harvesting a supernatant containing the retrovirus particle from the culture medium, wherein the culture medium contains either 100 nM to 3 μM trichostatin A or…
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