Reverse transcriptases and uses thereof
US-12065645-B2 · Aug 20, 2024 · US
Method of adaptor-dimer subtraction using a CRISPR CAS6 protein
US9102936B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9102936-B2 |
| Application number | US-201313894878-A |
| Country | US |
| Kind code | B2 |
| Filing date | May 15, 2013 |
| Priority date | Jun 11, 2012 |
| Publication date | Aug 11, 2015 |
| Grant date | Aug 11, 2015 |
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Abstract
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A method of processing a target RNA is provided. In certain embodiments, this method comprises: contacting the products of an RNA ligase-mediated ligation reaction with an CAS6 protein, wherein: (i) the RNA ligase-mediated ligation reaction comprises: a target RNA, an RNA ligase, and first and second adaptors that can ligate together to produce an adaptor dimer that contains a CRISPR stem loop; and (ii) the CAS6 protein recognizes the CRISPR stem loop; thereby preventing the adaptor dimer from being reverse transcribed.
First claim
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The invention claimed is: 1. A method of processing a target RNA, comprising: contacting the products of an RNA ligase-mediated ligation reaction with a CAS6 protein, wherein: (i) said RNA ligase-mediated ligation reaction comprises: a target RNA, an RNA ligase, and first and second adaptors that can ligate together to produce an adaptor dimer that contains a CRISPR stem loop; and (ii) said CAS6 protein recognizes said CRISPR stem loop; thereby preventing said adaptor dimer from…
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- C12N15/1096Primary
Chemistry & Metallurgy · mapped topic
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- What does patent US9102936B2 cover?
- A method of processing a target RNA is provided. In certain embodiments, this method comprises: contacting the products of an RNA ligase-mediated ligation reaction with an CAS6 protein, wherein: (i) the RNA ligase-mediated ligation reaction comprises: a target RNA, an RNA ligase, and first and second adaptors that can ligate together to produce an adaptor dimer that contains a CRISPR stem loop;…
- Who is the assignee on this patent?
- Agilent Technologies Inc
- What technology area does this patent fall under?
- Primary CPC classification C12N15/1096. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Aug 11 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).