Fermentation product containing equol-producing microorganism having maintained equol-producing ability, and method for producing same

Patent metadata

Field	Value
Publication number	US-9101159-B2
Application number	US-200913119353-A
Country	US
Kind code	B2
Filing date	Sep 18, 2009
Priority date	Sep 19, 2008
Publication date	Aug 11, 2015
Grant date	Aug 11, 2015

Abstract

An object of the present invention is to provide a fermented product containing equol-producing microorganisms in the state of living cells by which equol production ability is maintained.
When producing a fermented material by using an equol-producing microorganism, with soybean powder or soybean milk as raw materials, (1) preparing a mother starter by fermentation under anaerobic conditions by using an equol-producing microorganism in the presence of a daidzein species at pH 5.0 or higher, (2) preparing a bulk starter by fermentation under anaerobic conditions by using said mother starter in the presence of a daidzein species at pH 5.0 or higher, and (3) preparing a fermented material by fermentation by using said bulk starter in a medium containing soybean powder or soybean milk, enables production of a fermented material containing microorganisms in the state of living cells in which the equol production ability is maintained.

First claim

The invention claimed is:

1. A method for maintaining the equol production ability of an equol-producing microorganism in a fermented product, the method comprising:
adding at least one member selected from the group consisting of ascorbic acid, derivatives thereof, and salts thereof to a composition containing an equol-producing microorganism in need of such maintaining, in the state of a living cell,
wherein the at least one member is added to the composition at a proportion of 0.1 to 5 wt %.

2. The method according to claim 1 , wherein the pH of the composition is 5.0 or lower.

3. The method according to claim 1 , wherein the microorganism is contained at a concentration of 1×10 5 to 10 10 cfu/g in the state of living cell.

4. The method according to claim 1 , wherein the composition is a fermented food.

5. The method according to claim 4 , wherein the fermented food is a fermented soybean beverage or a fermented soybean milk.

6. The method according to claim 4 , which fermented food is stored at 10° C. or lower.

7. The method according to claim 1 , wherein the equol-producing microorganism is a lactic acid bacterium.

8. The method according to claim 7 , wherein the equol-producing microorganism is Lactococcus garvieae.

9. A method for producing a fermented product containing an equol-producing microorganism, the method comprising:
preparing a composition containing an equol-producing microorganism, in the state of a living cell; and adding to the composition an effective amount of at least one member selected from the group consisting of ascorbic acid, derivatives thereof, and salts thereof for maintaining the equol-producing ability of the microorganism in the fermented product
wherein the at least one member is added to the composition at a proportion of 0.1 to 5 wt %.

10. The method according to claim 9 , wherein the pH of the composition is 5.0 or lower.

11. The method according to claim 9 , wherein the composition is a fermented food.

12. The method according to claim 11 , wherein the fermented food is a fermented soybean beverage or a fermented soybean milk.

13. The method according to claim 11 , which fermented food is stored at 10° C. or lower.

Assignees

• Isono Yoshikazu
• Mori Hisako
• Ueno Tomomi
• Endo Rieko
• Kumemura Megumi
• Abiru Yasuhiro
• Uchiyama Shigeto
• Otsuka Pharma Co Ltd

Inventors

• Isono Yoshikazu
• Mori Hisako
• Ueno Tomomi
• Endo Rieko
• Kumemura Megumi
• Abiru Yasuhiro
• Uchiyama Shigeto

Classifications

• A61P3/06

  Antihyperlipidemics · CPC title

• A61P25/00

  Drugs for disorders of the nervous system · CPC title

• A61P19/10

  for osteoporosis · CPC title

• A23L33/135

  Bacteria or derivatives thereof, e.g. probiotics · CPC title

• A23C9/1236 Primary

  using Leuconostoc, Pediococcus or Streptococcus sp. other than Streptococcus Thermophilus; Artificial sour buttermilk in general (A23C9/1234, A23C17/02 take precedence; preservation of buttermilk or buttermilk preparations containing, or treated with, microorganisms or enzymes A23B11/55) · CPC title