Cell-matrix microspheres, methods for preparation and applications
US-9464271-B2 · Oct 11, 2016 · US
US9096830B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9096830-B2 |
| Application number | US-201113093606-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 25, 2011 |
| Priority date | Apr 23, 2010 |
| Publication date | Aug 4, 2015 |
| Grant date | Aug 4, 2015 |
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Multi-layered hydrogel constructs comprising: a first layer comprising a first polymerizable material, hyaluronic acid and a plurality of cells; a second layer comprising a second polymerizable material, chondroitin sulfate and a plurality of cells; and a third layer comprising a third polymerizable material, chondroitin sulfate, a matrix metalloprotease sensitive peptide, and a plurality of cells are provided. Associated methods are also provided.
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What is claimed is: 1. A multi-layered hydrogel construct comprising: a first layer comprising a first polymerizable material, hyaluronic acid and a plurality of cells; a second layer comprising a second polymerizable material, chondroitin sulfate and a plurality of cells; and a third layer comprising a third polymerizable material, chondroitin sulfate, a matrix metalloprotease sensitive peptide, and a plurality of cells. 2. The hydrogel construct of cla…
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