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Single molecule imaging techniques to aid crystallization
US9080979B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9080979-B2 |
| Application number | US-201214111950-A |
| Country | US |
| Kind code | B2 |
| Filing date | Apr 14, 2012 |
| Priority date | Apr 15, 2011 |
| Publication date | Jul 14, 2015 |
| Grant date | Jul 14, 2015 |
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Abstract
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This invention relates to a method to rapidly find crystallization conditions for a biomolecule in a desired conformation using single-molecule, fluorescent resonance energy transfer (smFRET) imaging techniques. The method provides significant cost and time advantages over the empricial exploration for crystallization conditions.
First claim
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We claim: 1. A method for selecting a crystallization for a biomolecule comprising: (a) labeling the biomolecule with a donor and acceptor fluorophore sufficiently spaced to report time-dependent dynamics of multiple conformations of the biomolecule by single molecule fluorescence resonance energy transfer (smFRET), wherein said multiple conformations includes a first conformation; (b) placing the labeled biomolecule under different crystallization conditions; (c) detecting fl…
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Chemistry & Metallurgy · mapped topic
- G01N21/6486Primary
Physics · mapped topic
- G01N33/542Primary
Physics · mapped topic
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Frequently asked questions
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- What does patent US9080979B2 cover?
- This invention relates to a method to rapidly find crystallization conditions for a biomolecule in a desired conformation using single-molecule, fluorescent resonance energy transfer (smFRET) imaging techniques. The method provides significant cost and time advantages over the empricial exploration for crystallization conditions.
- Who is the assignee on this patent?
- Blanchard Scott C, Feldman Michael Brian, Wang Leyi, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification G01N21/6486. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Jul 14 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).