Apparatus and methods for integrated sample preparation, reaction and detection

Patent metadata

Field	Value
Publication number	US-9074250-B2
Application number	US-201313759557-A
Country	US
Kind code	B2
Filing date	Feb 5, 2013
Priority date	Oct 19, 2005
Publication date	Jul 7, 2015
Grant date	Jul 7, 2015

Abstract

Cartridges for the isolation of a biological sample and downstream biological assays on the sample are provided, as are methods for using such cartridges. In one embodiment, a nucleic acid sample is isolated from a biological sample and the nucleic acid sample is amplified, for example by the polymerase chain reaction. The cartridges provided herein can also be used for the isolation of non-nucleic acid samples, for example proteins, and to perform downstream reactions on the proteins, for example, binding assays. Instruments for carrying out the downstream biological assays and for detecting the results of the assays are also provided.

First claim

What is claimed is:

1. A method comprising:
(a) providing a composition comprising nucleic acids bound to magnetic particles in a first chamber of a cassette comprising the first chamber and a reaction chamber;
(b) eluting the nucleic acids from the particles in the first chamber to obtain an eluted nucleic acid sample;
(c) magnetically attracting the magnetic particles to a cavity of a valve member movably disposed within a valve housing, wherein the valve housing is at least partially disposed between the first chamber and a second chamber and comprises a first opening in fluid communication with the first chamber and a second opening in fluid communication with the second chamber;
(d) transferring at least a portion of the eluted nucleic acid sample to the reaction chamber in fluid communication with the first chamber;
(e) amplifying one or more target nucleic acid sequences in the eluted nucleic acid sample by combining the eluted nucleic acid sample and PCR reagents in the reaction chamber under conditions suitable for PCR.

2. The method of claim 1 , wherein the nucleic acids are eluted in an elution buffer comprising MgCl 2 .

3. The method of claim 1 , wherein one or more of the PCR reagents are provided in lyophilized form in the reaction chamber such that the PCR reagents are reconstituted upon transfer of the eluted nucleic acid sample to the reaction chamber.

4. The method of claim 1 , further comprising heating the eluted nucleic acid sample to above 70° C. prior to transferring the eluted nucleic acid sample to the reaction chamber.

5. The method of claim 1 , further comprising heating the eluted nucleic acid sample to above 95° C. prior to transferring the eluted nucleic acid sample to the reaction chamber.

6. The method of claim 1 , wherein transferring the eluted nucleic acid sample to the reaction chamber comprises applying negative pressure to the first chamber.

7. The method of claim 1 , further comprising monitoring the PCR in real time.

8. The method of claim 1 , wherein the particles are magnetic silica particles.

9. The method of claim 1 further comprising removing the magnetic particles from the first chamber while retaining substantially all of the eluted nucleic acid sample in the first chamber by moving the valve member from a first position in which the cavity is in fluid communication with the first chamber to a second position in which the cavity is fluidically isolated from the first chamber.

10. A method comprising:
(a) providing a composition comprising nucleic acids bound to magnetic particles in a first chamber of a cassette comprising the first chamber and a reaction chamber;
(b) eluting the nucleic acids from the particles in the first chamber to obtain an eluted nucleic acid sample;
(c) magnetically attracting the magnetic particles to a cavity of a valve member movably disposed within a valve housing, wherein the valve housing is at least partially disposed between the first chamber and a second chamber and comprises a first opening in fluid communication with the first chamber and a second opening in fluid communication with the second chamber;
(d) transferring at least a portion of the eluted nucleic acid sample to the reaction chamber in fluid communication with the first chamber; and
(e) amplifying one or more target nucleic acid sequences in the eluted nucleic acid sample by combining the eluted nucleic acid sample and PCR reagents in the reaction chamber under conditions suitable for PCR,
wherein a PCR master mix containing PCR reagents and substantially no MgCl 2 is disposed in the reaction chamber, and the nucleic acids are eluted in an elution buffer comprising MgCl 2 .

11. The method of claim 10 , wherein one or more of the PCR reagents are provided in lyophilized form in the reaction chamber such that the PCR reagents are reconstituted upon transfer of the eluted nucleic acid sample to the reaction chamber.

12. The method of claim 10 , further comprising heating the eluted nucleic acid sample to above 70° C. prior to transferring the eluted nucleic acid sample to the reaction chamber.

13. The method of claim 10 , further comprising heating the eluted nucleic acid sample to above 95° C. prior to transferring the eluted nucleic acid sample to the reaction chamber.

14. The method of claim 10 , wherein transferring the eluted nucleic acid sample to the reaction chamber comprises applying negative pressure to the first chamber.

15. The method of claim 10 , further comprising monitoring the PCR in real time.

16. The method of claim 10 further comprising removing the magnetic particles from the first chamber while retaining substantially all of the eluted nucleic acid sample in the first chamber by moving the valve member from a first position in which the cavity is in fluid communication with the first chamber to a second position in which the cavity is fluidically isolated from the first chamber.

Assignees

• Luminex Corp

Inventors

• Bird Dylan Hilmer
• Ching Jesus
• Johnson Bruce A
• Moravick Keith E
• Richardson Bruce

Classifications

• G01N1/38

  Diluting, dispersing or mixing samples · CPC title

• B01L2400/0633

  with moving parts · CPC title

• B01L2300/042

  Caps; Plugs · CPC title

• B01L2200/025

  Align devices or objects to ensure defined positions relative to each other · CPC title

• B01L2200/026

  Fluid interfacing between devices or objects, e.g. connectors, inlet details · CPC title