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Methods and compositions for a soybean in-planta transient expression system
US9068191B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9068191-B2 |
| Application number | US-201113326786-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 15, 2011 |
| Priority date | Dec 29, 2010 |
| Publication date | Jun 30, 2015 |
| Grant date | Jun 30, 2015 |
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Abstract
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This invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed; (b2) applying a vacuum to the immersed soybean plant and/or intact part thereof of step (b1); and (b3) releasing the vacuum, thereby introducing the solution into the plant and/or intact part thereof; or (c) contacting the abraded plant and/or abraded intact part thereof with a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nucleotide sequence to be transiently expressed, whereby the nucleotide sequence is transiently expressed in one or more cells of the plant.
First claim
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What is claimed is: 1. A method of transiently expressing one or more nucleotide sequences in one or more cells of an intact whole soybean plant, comprising: a) contacting the surface of an intact soybean leaf of a whole soybean plant with an abrasive; b) contacting the abraded soybean leaf and/or the whole soybean plant comprising the leaf with a solution comprising bacterial cells competent for nucleic acid transfer and a surfactant, wherein the bacterial cells comprise the on…
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- C12N15/8205Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9068191B2 cover?
- This invention provides methods of transiently expressing a nucleotide sequence in one or more cells of a soybean plant, comprising: a) abrading the surface of a soybean plant and/or intact part thereof; and either (b1) immersing the abraded plant and/or abraded intact part thereof in a solution comprising Agrobacterium cells and a surfactant, wherein the Agrobacterium cells comprise the nu…
- Who is the assignee on this patent?
- Azhakanandam Kasimalai, Su Pei, Syngenta Participations Ag
- What technology area does this patent fall under?
- Primary CPC classification C12N15/8205. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 30 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).