Optogenetic probes for measuring membrane potential
US-10800829-B2 · Oct 13, 2020 · US
Bacteriorhodopsin fusion membrane protein expression system
US9067985B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9067985-B2 |
| Application number | US-201314041493-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 30, 2013 |
| Priority date | Oct 6, 2012 |
| Publication date | Jun 30, 2015 |
| Grant date | Jun 30, 2015 |
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Abstract
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An expression vector is disclosed, which comprises: a) a polynucleotide sequence encoding a bacteriorhodopsin or a mutant bacteriorhodopsin; b) a multiple cloning site; c) a T7 promoter, d) a polyhistidine tag; e) a first protease cleavage site; f) optionally a second protease cleavage site; and g) optionally a linker; wherein the mutant bacteriorhodopsin comprises the residue corresponding to Asn94 of SEQ ID NO: 1. Also disclosed is a fusion membrane protein expression system, which comprises: a) a polynucleotide sequence encoding a mutant Haloarcula marismortui bacteriorhodopsin/D94N (HmBRI/D94N) or a Haloquadratum walsbyi bacteriorhodopsin (HwBR); b) a target membrane protein; and c) a T7 promoter, operably linked to the mutant HmBRI/D94N or HwBR and the target membrane protein. Host cells comprising the expression vector or the fusion membrane protein expression system and methods of using the same are also disclosed.
First claim
Opening claim text (preview).
What is claimed is: 1. An expression vector comprising: a) a polynucleotide sequence encoding a mutant bacteriorhodopsin having at least 80% sequence identity to SEQ ID NO: 1; b) a multiple cloning site, located downstream from the polynucleotide encoding the mutant bacteriorhodopsin; c) a T7 promoter, operably linked to the polynucleotide sequence encoding the mutant bacteriorhodopsin, d) a nucleic acid sequence encoding a polyhistidine tag, located downstream from the poly…
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- C07K14/215Primary
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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- What does patent US9067985B2 cover?
- An expression vector is disclosed, which comprises: a) a polynucleotide sequence encoding a bacteriorhodopsin or a mutant bacteriorhodopsin; b) a multiple cloning site; c) a T7 promoter, d) a polyhistidine tag; e) a first protease cleavage site; f) optionally a second protease cleavage site; and g) optionally a linker; wherein the mutant bacteriorhodopsin comprises the residue corresponding to …
- Who is the assignee on this patent?
- Academia Sinica
- What technology area does this patent fall under?
- Primary CPC classification C07K14/215. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Jun 30 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).