Methods for Nucleic Acid Cleavage
US-2024417778-A1 · Dec 19, 2024 · US
US9051601B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9051601-B2 |
| Application number | US-83236707-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 1, 2007 |
| Priority date | Aug 1, 2006 |
| Publication date | Jun 9, 2015 |
| Grant date | Jun 9, 2015 |
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Official abstract text for this publication.
Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.
Opening claim text (preview).
We claim: 1. A method for isolating nucleic acid from a sample, comprising the steps of: a. mixing a sample containing nucleic acids with a non-specific capture probe comprising a first region that is a randomized poly(k) sequence at a length of 18 residues comprising G and T nucleotides or G and U nucleotides attached to a second region that is a first specific binding partner (SBP); b. incubating a reaction mixture containing a support and the mixture of nucleic acids and non-…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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