Methods of nonspecific target capture of nucleic acids

US9051601B2 · US · B2

Patent metadata
FieldValue
Publication numberUS-9051601-B2
Application numberUS-83236707-A
CountryUS
Kind codeB2
Filing dateAug 1, 2007
Priority dateAug 1, 2006
Publication dateJun 9, 2015
Grant dateJun 9, 2015

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Abstract

Official abstract text for this publication.

Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic acid and specifically to an immobilized probe via binding of members of a specific binding pair in a solution phase of a reaction mixture are disclosed.

First claim

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We claim: 1. A method for isolating nucleic acid from a sample, comprising the steps of: a. mixing a sample containing nucleic acids with a non-specific capture probe comprising a first region that is a randomized poly(k) sequence at a length of 18 residues comprising G and T nucleotides or G and U nucleotides attached to a second region that is a first specific binding partner (SBP); b. incubating a reaction mixture containing a support and the mixture of nucleic acids and non-…

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What does patent US9051601B2 cover?
Methods for capturing a target nucleic acid from a sample by using a capture probe that binds nonspecifically to the target nucleic acid and binds specifically to an immobilized probe via a specific binding pair that has one member on the capture probe and one member on the immobilized probe are disclosed. Compositions that include a capture probe that binds nonspecifically to a target nucleic …
Who is the assignee on this patent?
Becker Michael M, Majlessi Mehrdad R, Gen Probe Inc
What technology area does this patent fall under?
Primary CPC classification C12Q1/6806. Mapped technology areas include Chemistry & Metallurgy.
When was this patent published?
Publication date Tue Jun 09 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
What related patents are in patentsdb?
We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).