Soybean markers linked to SCN resistance

Patent metadata

Field	Value
Publication number	US-9049822-B2
Application number	US-201113287819-A
Country	US
Kind code	B2
Filing date	Nov 2, 2011
Priority date	Nov 5, 2010
Publication date	Jun 9, 2015
Grant date	Jun 9, 2015

Abstract

This disclosure concerns compositions and methods for identifying the SCN resistant phenotype in soybean. In some embodiments, the disclosure concerns methods for performing marker-assisted breeding and selection of plants carrying one or more determinants of SCN resistance in soybean.

First claim

What may be claimed is:

1. A method for identifying a plant comprising at least one determinant of SCN resistance in a soybean variety, the method comprising:
isolating nucleic acid molecules from a plant of the soybean variety; and
screening the isolated nucleic acid molecules for a marker linked to the SCN resistance phenotype in the soybean variety, wherein the marker has at least 90% sequence identity to SEQ ID NO:10, and the presence of the marker is indicative of having SCN resistance in the soybean variety.

2. The method according to claim 1 , wherein the marker linked to the SCN resistance phenotype in the soybean variety is in soybean linkage group G.

3. The method according to claim 1 , wherein the isolated nucleic acid molecules are genomic DNA.

4. The method according to claim 1 , wherein the SCN resistance phenotype is SCN resistance to SCN race 3.

5. The method according to claim 1 , wherein the marker linked to the SCN resistance phenotype in the soybean variety is SEQ ID NO:10.

6. The method according to claim 1 , wherein screening the isolated nucleic acid molecules for a marker linked to the SCN resistance phenotype in the soybean variety is performed using competitive allele-specific polymerase chain reaction.

7. The method according to claim 1 , further comprising determining the genotype of the plant for the marker linked to the SCN resistance phenotype in the soybean variety.

8. A method for producing an SCN resistant soybean plant, the method comprising:
crossing a soybean plant having the trait of SCN resistance with a soybean plant from a soybean variety of interest;
using marker-assisted selection to identify an F 1 soybean plant comprising a marker linked to the SCN resistance phenotype in the soybean plant having the trait of SCN resistance, wherein the marker has at least 90% sequence identity to SEQ ID NO:10 and the F 1 soybean plant has any desirable traits of the soybean variety of interest; and
propagating the identified F 1 soybean plant, thereby producing an SCN resistant soybean plant.

9. The method according to claim 8 , wherein the marker linked to the SCN resistance phenotype in the soybean plant having the trait of SCN resistance is in linkage group G.

10. The method according to claim 8 , wherein the soybean variety of interest is an SCN susceptible soybean variety.

11. The method according to claim 8 , wherein the SCN resistance is resistance to SCN race 3.

12. The method according to claim 8 , wherein the soybean plant having the trait of SCN resistance is a soybean plant of variety 98860-71.

13. The method according to claim 12 , wherein the marker linked to the SCN resistance phenotype in the soybean plant having the trait of SCN resistance or equivalent thereof is SEQ ID NO:10.

14. The method according to claim 8 , wherein marker-assisted selection is performed using competitive allele-specific polymerase chain reaction.

15. A method for introducing SCN resistance to a soybean plant, the method comprising introducing a marker linked to a SCN resistance phenotype into an SCN susceptible soybean plant, wherein the marker linked to the SCN resistance phenotype in soybean variety 98860-71 has at least 90% sequence identity to SEQ ID NO:10.

Assignees

• Bai Yonghe
• Lu Fang
• Greene Thomas W
• Moore Robert E
• Hedges Bradley
• Kumpatla Siva P
• Ram Raghav
• Agrigenetics Inc

Inventors

• Bai Yonghe
• Lu Fang
• Greene Thomas W
• Moore Robert E
• Hedges Bradley
• Kumpatla Siva P
• Ram Raghav

Classifications

• C12Q2600/13

  Plant traits · CPC title

• A01H1/04 Primary

  Processes of selection {involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection} · CPC title

• C12Q1/6895 Primary

  for plants, fungi or algae · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• A01H5/00

  Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy · CPC title