Non-viral delivery of compounds to mitochondria

Patent metadata

Field	Value
Publication number	US-9045552-B2
Application number	US-201113082526-A
Country	US
Kind code	B2
Filing date	Apr 8, 2011
Priority date	Oct 24, 2003
Publication date	Jun 2, 2015
Grant date	Jun 2, 2015

Abstract

A conjugate comprises: (a) a mitochondrial membrane-permeant peptide; (b) an active agent or compound of interest such as a detectable group or mitochondrial protein or peptide; and (c) a mitochondrial targeting sequence linking said mitochondrial membrane-permeant peptide and said active mitochondrial protein or peptide. The targeting sequence is one which is cleaved within the mitochondrial matrix, and not cleaved within the cellular cytoplasm, of a target cell into which said compound is delivered. Methods of use of such compounds are also described.

First claim

That which is claimed is:

1. A conjugate, comprising:
(a) a HIV-TAT protein transduction domain peptide comprising at least about four sequential amino acid residues of SEQ ID NO:1;
(b) an active mitochondrial protein or peptide; and
(c) a mitochondrial targeting sequence linking said protein transduction domain peptide and said active mitochondrial protein or peptide, wherein said targeting sequence is cleaved within the mitochondrial matrix and not cleaved within the cellula…

Assignees

• Payne R Mark
• Univ Wake Forest

Inventors

• Payne R Mark

Classifications

• A61P3/00

  Human Necessities · mapped topic

• A61K38/00

  Human Necessities · mapped topic

• A61P21/00

  Human Necessities · mapped topic

• C07K14/47 Primary

  Chemistry & Metallurgy · mapped topic

• C07K2319/10

  Chemistry & Metallurgy · mapped topic