Biomolecule fixing board and method of manufacturing the same

Patent metadata

Field	Value
Publication number	US-9040251-B2
Application number	US-201013388671-A
Country	US
Kind code	B2
Filing date	Jul 29, 2010
Priority date	Aug 4, 2009
Publication date	May 26, 2015
Grant date	May 26, 2015

Abstract

This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.

First claim

The invention claimed is:

1. A biomolecule modifying substrate comprising:
a substrate;
a first surface on the substrate, the first surface comprising a metal oxide;
a second surface on the substrate, the second surface comprising a noble metal;
a first linker molecule comprising a hydrocarbon chain and a functional group at one end or in a side chain of the hydrocarbon chain, the functional group capable of selectively binding to the first surface, the first linker molecule bound to the first surface via the functional group;
a second linker molecule comprising biotin and a reactive group, the reactive group capable of binding to the hydrocarbon chain of the first linker molecule, the second linker molecule bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain;
a biomolecule comprising an introduced biotin; and
an avidin,
wherein the biotin contained in the second linker molecule is bound to the biotin introduced into the biomolecule via the avidin, and
wherein the functional group of the first linker molecule capable of selectively binding to the first surface is a phosphate group.

2. The biomolecule modifying substrate according to claim 1 , wherein the first surface is a metal having a natural oxide film.

3. The biomolecule modifying substrate according to claim 1 , wherein the hydrocarbon chain of the first linker molecule comprises an alkyl group and is represented by the formula selected from the group consisting of C m H 2m-1 PO 3 H 2 , C m H 2m-1 OPO 3 H 2 , wherein m is an integer between 2 and 20.

4. The biomolecule modifying substrate according to claim 1 , wherein the hydrocarbon chain of the first linker molecule is a polymer chain comprising a phosphate group in the side chain of a compound represented by formula 3:

wherein n is an integer of 1 or larger.

5. The biomolecule modifying substrate according to claim 1 , wherein the reactive group in the second linker molecule is a latent reactive group capable of binding to the hydrocarbon chain and the latent reactive group is at least 1 type of photoreactive compound selected from the group consisting of an anthrathione group, an anthraquinone group, a benzophenone group, an azide group, and a derivative of any thereof.

6. The biomolecule modifying substrate according to claim 1 , wherein the second linker molecule further comprises a reactive group capable of selectively binding to the biomolecule or a reactive group bound to the biomolecule.

7. The biomolecule modifying substrate according to claim 1 ,
wherein the first linker molecule lacks a functional group inhibiting binding between the second linker molecule and the hydrocarbon chain of the first linker molecule, and
wherein the first linker molecule lacks a functional group capable of binding to the second surface.

8. The biomolecule modifying substrate according to claim 1 ,
wherein the hydrocarbon chain of the first linker molecule comprises an alkyl group, and
wherein functional group of the second linker molecule is bonded to the alkyl group of the first linker molecule.

Assignees

• Tada Yasuhiko
• Yoshida Hiroshi
• Saito Toshiro
• Narahara Masatoshi
• Nakagawa Hiroaki
• Hitachi High Tech Corp

Inventors

• Tada Yasuhiko
• Yoshida Hiroshi
• Saito Toshiro
• Narahara Masatoshi
• Nakagawa Hiroaki

Classifications

• G01N33/54353 Primary

  with ligand attached to the carrier via a chemical coupling agent (coatings G01N33/54393) · CPC title

• G01N33/54393

  Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding · CPC title

• G01N33/543

  with an insoluble carrier for immobilising immunochemicals · CPC title

• A61K2039/625

  binding through the biotin-streptavidin system or similar · CPC title