Multiplex nucleic acid amplification
US-2015361481-A1 · Dec 17, 2015 · US
US9040243B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9040243-B2 |
| Application number | US-201113825226-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 22, 2011 |
| Priority date | Sep 23, 2010 |
| Publication date | May 26, 2015 |
| Grant date | May 26, 2015 |
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The present invention relates to a method, kit and use of various nucleic acid sequences for deleting and/or quantifying one or more nucleic acids of a genome in a sample. Wherein the nucleic acid is amplified and the locus that is amplified is a multi copy locus within the genome, the multicopy locus has copies on at least two different chromosomes and the amplification product is detected and/or quantified.
Opening claim text (preview).
The invention claimed is: 1. A method of quantifying one or more nucleic acids of a genome in a sample comprising: a. amplifying a nucleic acid comprising a non-repetitive, multicopy locus, the locus having copies on at least 2 chromosomes and a sequence that shares at least 80% sequence identity over a stretch of 80 base pairs to a nucleic acid sequence of SEQ ID NO.1 or SEQ ID NO.52 to produce an amplification product; b. detecting the amplification product; and c. quantifyi…
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
Chemistry & Metallurgy · mapped topic
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