Quantitative pcr method using internal control
US-2024368681-A1 · Nov 7, 2024 · US
Dried composition for hot-start PCR with long-term stability
US9034603B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9034603-B2 |
| Application number | US-68245608-A |
| Country | US |
| Kind code | B2 |
| Filing date | Oct 28, 2008 |
| Priority date | Oct 29, 2007 |
| Publication date | May 19, 2015 |
| Grant date | May 19, 2015 |
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Abstract
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The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl 2 , 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.
First claim
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What is claimed is: 1. A dried composition in a reaction tube for hot-start PCR comprising reaction buffer, MgCl 2 , 4 types of dNTPs, DNA polymerase, pyrophosphate, pyrophosphatase which converts inorganic pyrophosphate to two phosphate ions, and one or more stabilizer selected from the group consisting of polyol, gelatin, bovine serum albumin (BSA), Thesit, and PET-8000. 2. The dried composition in a reaction tube for hot-start PCR according to claim 1 , wher…
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- What does patent US9034603B2 cover?
- The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl 2 , 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase…
- Who is the assignee on this patent?
- Kim Seong-Youl, Kim Hyun Bae, Park Hae-Joon, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12Q1/686. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue May 19 2015 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).