Method for preparing porous scaffold for tissue engineering

Patent metadata

Field	Value
Publication number	US-9028857-B2
Application number	US-68167608-A
Country	US
Kind code	B2
Filing date	Oct 10, 2008
Priority date	Oct 11, 2007
Publication date	May 12, 2015
Grant date	May 12, 2015

Abstract

The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprise the steps consisting of a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide and one cross-linking agent b) freezing the aqueous solution of step a) c) sublimating the frozen solution of step b). characterized in that step b) is performed before the cross-linking of the polysaccharide occurs in the solution of step a).

First claim

The invention claimed is:

1. A method for preparing a porous scaffold consisting of the steps of
a) preparing an alkaline aqueous solution consisting of an amount of at least one natural polysaccharide and one cross-linking agent;
b) freezing the aqueous solution of step a); and
c) sublimating the frozen solution of step b);
wherein the at least one natural polysaccharide is selected from the group consisting of dextran, agar, alginic acid, hyaluronic acid, inulin, pullulan, heparin, chitonsan, and fucoidan; and
wherein step b) is performed before the cross-linking of the natural polysaccharide and said cross-linking occurs during the sublimation step c).

2. The method according to claim 1 wherein said cross-linking agent is selected in the group consisting of trisodium trimetaphosphate (STMP), phosphorus oxychloride (POCl3), eupichlorohydrin, formaldehydes, water soluble carbodiimides, and glutaraldehydes.

3. The method according to claim 2 wherein said cross-linking agent is trisodium trimetaphosphate (STMP).

4. The method according to claim 1 wherein the aqueous solution of step a) is freeze-dried.

5. The method according to claim 4 wherein the aqueous solution of step a) is freeze-dried under a pressure from 0.1 mBar to 6.5 mBar.

6. The method according to claim 1 wherein the aqueous solution of step a) is poured in a mould before step b).

7. The method according to claim 1 wherein said scaffold is shaped.

Assignees

• Le Visage Catherine
• Letourneur Didier
• Chaubet Frederic
• Autissier Aude
• Inst Nat Sante Rech Med
• Univ Paris 7—Denis Diderot

Inventors

• Le Visage Catherine
• Letourneur Didier
• Chaubet Frederic
• Autissier Aude

Classifications

• Y10T436/147777

  Plural nitrogen in the same ring [e.g., barbituates, creatinine, etc.] · CPC title

• C12N11/10

  the carrier being a carbohydrate · CPC title

• C08J3/24

  Crosslinking, e.g. vulcanising, of macromolecules (mechanical aspects B29C35/00; crosslinking agents C08K) · CPC title

• A61L2430/06

  for cartilage reconstruction, e.g. meniscus · CPC title

• A61L2300/21

  Acids · CPC title